{PDOC00071}
{PS00074; GLFV_DEHYDROGENASE}
{BEGIN}
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* Glu / Leu / Phe / Val dehydrogenases active site *
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 - Glutamate  dehydrogenases  (EC 1.4.1.2, EC 1.4.1.3, and EC 1.4.1.4) (GluDH)
   are enzymes that catalyze the NAD- or NADP-dependent reversible deamination
   of glutamate into  alpha-ketoglutarate [1,2]. GluDH isozymes are  generally
   involved with either ammonia assimilation or glutamate catabolism.
 - Leucine dehydrogenase (EC 1.4.1.9) (LeuDH) is a  NAD-dependent enzyme  that
   catalyzes the reversible deamination of leucine and several other aliphatic
   amino acids to their keto analogues [3].
 - Phenylalanine dehydrogenase (EC 1.4.1.20) (PheDH) is a NAD-dependent enzyme
   that catalyzes the  reversible deamidation of  L-phenylalanine into phenyl-
   pyruvate [4].
 - Valine  dehydrogenase  (EC 1.4.1.8) (ValDH) is a NADP-dependent enzyme that
   catalyzes the  reversible   deamidation   of   L-valine   into  3-methyl-2-
   oxobutanoate [5].

These  dehydrogenases  are structurally and functionally related.  A conserved
lysine  residue located in a  glycine-rich  region has been  implicated in the
catalytic mechanism. The conservation of the region around this residue allows
the derivation of a signature pattern for such type of enzymes.

-Consensus pattern: [LIV]-x(2)-G-G-[SAG]-K-x-[GV]-x(3)-[DNST]-[PL]
                    [K is the active site residue]
-Sequences known to belong to this class detected by the pattern: ALL.
-Other sequence(s) detected in Swiss-Prot: NONE.

-Note: All known sequences from this family have Pro in the  last  position of
 the pattern with the exception of yeast GluDH which as Leu.

-Last update: November 1997 / Pattern and text revised.

[ 1] Britton K.L., Baker P.J., Rice D.W., Stillman T.J.
     "Structural relationship between the hexameric and tetrameric family
     of glutamate dehydrogenases."
     Eur. J. Biochem. 209:851-859(1992).
     PubMed=1358610
[ 2] Benachenhou-Lahfa N., Forterre P., Labedan B.
     J. Mol. Evol. 36:335-346(1993).
[ 3] Nagata S., Tanizawa K., Esaki N., Sakamoto Y., Ohshima T., Tanaka H.,
     Soda K.
     "Gene cloning and sequence determination of leucine dehydrogenase from
     Bacillus stearothermophilus and structural comparison with other
     NAD(P)+-dependent dehydrogenases."
     Biochemistry 27:9056-9062(1988).
     PubMed=3069133
[ 4] Takada H., Yoshimura T., Ohshima T., Esaki N., Soda K.
     "Thermostable phenylalanine dehydrogenase of Thermoactinomyces
     intermedius: cloning, expression, and sequencing of its gene."
     J. Biochem. 109:371-376(1991).
     PubMed=1880121
[ 5] Tang L., Hutchinson C.R.
     "Sequence, transcriptional, and functional analyses of the valine
     (branched-chain amino acid) dehydrogenase gene of Streptomyces
     coelicolor."
     J. Bacteriol. 175:4176-4185(1993).
     PubMed=8320231

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