{PDOC00096} {PS00103; PUR_PYR_PR_TRANSFER} {BEGIN} *********************************************************** * Purine/pyrimidine phosphoribosyl transferases signature * *********************************************************** Phosphoribosyltransferases (PRT) are enzymes that catalyze the synthesis of beta-n-5'-monophosphates from phosphoribosylpyrophosphate (PRPP) and an enzyme specific amine. A number of PRT's are involved in the biosynthesis of purine, pyrimidine, and pyridine nucleotides, or in the salvage of purines and pyrimidines. These enzymes are: - Adenine phosphoribosyltransferase (EC 2.4.2.7) (APRT), which is involved in purine salvage. - Hypoxanthine-guanine or hypoxanthine phosphoribosyltransferase (EC 2.4.2.8) (HGPRT or HPRT), which are involved in purine salvage. - Orotate phosphoribosyltransferase (EC 2.4.2.10) (OPRT), which is involved in pyrimidine biosynthesis. - Amido phosphoribosyltransferase (EC 2.4.2.14), which is involved in purine biosynthesis. - Xanthine-guanine phosphoribosyltransferase (EC 2.4.2.22) (XGPRT), which is involved in purine salvage. In the sequence of all these enzymes there is a small conserved region which may be involved in the enzymatic activity and/or be part of the PRPP binding site [1]. -Consensus pattern: [LIVMFYWCTA]-[LIVM]-[LIVMA]-[LIVMFC]-[DE]-D-[LIVMS]- [LIVM]-[STAVD]-[STAR]-[GAC]-x-[STAR] -Sequences known to belong to this class detected by the pattern: ALL, except for Bacillus subtilis xanthine phosphoribosyltransferase. -Other sequence(s) detected in Swiss-Prot: bacterial phosphoribosyl pyro- phosphate synthases and 7 other proteins. -Note: In position 11 of the pattern most of these enzymes have Gly. -Last update: November 1997 / Pattern and text revised. [ 1] Hershey H.V., Taylor M.W. "Nucleotide sequence and deduced amino acid sequence of Escherichia coli adenine phosphoribosyltransferase and comparison with other analogous enzymes." Gene 43:287-293(1986). PubMed=3527873 -------------------------------------------------------------------------------- PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see https://prosite.expasy.org/prosite_license.html -------------------------------------------------------------------------------- {END}