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| PROSITE documentation PDOC00097 |
EPSP synthase signatures
Description:
EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase) (EC 2.5.1.19)
catalyzes the sixth step in the biosynthesis from chorismate of the aromatic
amino acids (the shikimate pathway) in bacteria (gene aroA), plants and fungi
(where it is part of a multifunctional enzyme which catalyzes five consecutive
steps in this pathway) [1]. EPSP synthase has been extensively studied as it
is the target of the potent herbicide glyphosate which inhibits the enzyme.
The sequence of EPSP from various biological sources shows that the structure
of the enzyme has been well conserved throughout evolution. We selected two
conserved regions as signature patterns. The first pattern corresponds to a
region that is part of the active site and which is also important for the
resistance to glyphosate [2]. The second pattern is located in the C-terminal
part of the protein and contains a conserved lysine which seems to be
important for the activity of the enzyme.
Last update:
April 2006 / Pattern revised.
Technical section:
PROSITE methods (with tools and information) covered by this documentation:
| EPSP_SYNTHASE_1, PS00104; EPSP synthase signature 1 (PATTERN) |
| Consensus pattern: |
[LIVF]-{LV}-x-[GANQK]-[NLG]-[SA]-[GA]-[TAI]-[STAGV]-{N}-R-x-[LIVMFYAT]-x-[GSTAP]
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| Sequences known to belong to this class detected by the pattern: |
ALL, except for Mycobacterium tuberculosis aroA |
| Other sequence(s) detected in Swiss-Prot: |
NONE. |
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| Matching PDB structures:
1EPS 1G6S 1G6T 1LN5 ... [ALL] |
| EPSP_SYNTHASE_2, PS00885; EPSP synthase signature 2 (PATTERN) |
| Consensus pattern: |
[KR]-x-[KH]-E-[CSTVI]-[DNE]-R-[LIVMY]-x-[GSTAVLD]-[LIVMCTF]-x(3)-[LIVMFA]-x(2)-[LIVMFCGANY]-G
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| Sequences known to belong to this class detected by the pattern: |
ALL, except for Lactococcus lactis and Staphylococcus aureus aroA |
| Other sequence(s) detected in Swiss-Prot: |
NONE. |
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| Matching PDB structures:
1EPS 1G6S 1G6T 1LN5 ... [ALL] |
References:
| 1 |
Authors | Stallings W.C., Abdel-Meguid S.S., Lim L.W., Shieh H.-S., Dayringer H.E., Leimgruber N.K., Stegeman R.A., Anderson K.S., Sikorski J.A., Padgette S.R., Kishore G.M. |
| Title | Structure and topological symmetry of the glyphosate target 5-enolpyruvylshikimate-3-phosphate synthase: a distinctive protein fold. |
| Source | Proc. Natl. Acad. Sci. U.S.A. 88:5046-5050(1991). |
| PubMed ID | 11607190 |
| 2 |
Authors | Padgette S.R., Re D.B., Gaser C.S., Eicholtz D.A., Frazier R.B., Hironaka C.M., Levine E.B., Shah D.M., Fraley R.T., Kishore G.M. |
| Title | Site-directed mutagenesis of a conserved region of the 5-enolpyruvylshikimate-3-phosphate synthase active site. |
| Source | J. Biol. Chem. 266:22364-22369(1991). |
| PubMed ID | 1939260 |
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