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3'5'-cyclic nucleotide phosphodiesterases (EC 3.1.4.17) (PDEases) catalyze the hydrolysis of cAMP or cGMP to the corresponding nucleoside 5' monophosphates [1]. There are at least seven different subfamilies of PDEases [2,E1]:

• Type 1, calmodulin/calcium-dependent PDEases.
• Type 2, cGMP-stimulated PDEases.
• Type 3, cGMP-inhibited PDEases.
• Type 4, cAMP-specific PDEases.
• Type 5, cGMP-specific PDEases.
• Type 6, rhodopsin-sensitive cGMP-specific PDEases.
• Type 7, High affinity cAMP-specific PDEases.

All of these forms seem to share a conserved domain of about 270 residues. We have derived a signature pattern from a stretch of 12 residues that contains two conserved histidines.

Slime mold extracellular PDEase and yeast low-affinity PDEase (gene PDE1) do not show any similarity with the above enzymes and belong to another class of PDEases (see <PDOC00530>).

July 1998 / Pattern and text revised.

PROSITE method (with tools and information) covered by this documentation:

PDEASE_I, PS00126; 3'5'-cyclic nucleotide phosphodiesterases signature  (PATTERN)

Consensus pattern: H-D-[LIVMFY]-x-H-x-[AG]-x(2)-[NQ]-x-[LIVMFY] Sequences known to belong to this class detected by the pattern: ALL Other sequence(s) detected in Swiss-Prot: NONE.

• Retrieve an alignment of Swiss-Prot true positive hits:   Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format • Retrieve the sequence logo from the alignment • Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS00126 • Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS00126 • Scan Swiss-Prot/TrEMBL entries against PS00126 • view ligand binding statistics

Matching PDB structures: 1F0J 1JP1 1JP2 1LHQ ... [ALL]

1	Authors	Charbonneau H., Beier N., Walsh K.A., Beavo J.A.
	Title	Identification of a conserved domain among cyclic nucleotide phosphodiesterases from diverse species.
	Source	Proc. Natl. Acad. Sci. U.S.A. 83:9308-9312(1986).
	PubMed ID	3025833

2	Authors	Beavo J.A., Reifsnyder D.H.
	Title	Primary sequence of cyclic nucleotide phosphodiesterase isozymes and the design of selective inhibitors.
	Source	Trends Pharmacol. Sci. 11:150-155(1990).
	PubMed ID	2159198

E1
Source	http://depts.washington.edu/pde/

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