Aspartyl proteases (APs), also known as acid proteases, (EC 3.4.23.-) are a
widely distributed family of proteolytic enzymes [1,2,3,4,5] known to exist in
vertebrates, fungi, plants, retroviruses and some plant viruses. APs use an
Asp dyad to hydrolyze peptide bonds.
APs found in eukaryotic cells are α/β monomers composed of two
asymmetric lobes, with the catalytic Asp dyad located at the lobe interface
and a flap made up of a β-hairpin covering the peptide substrates ("pepsin-like" proteases) (see <PDB:1FKN>). Each of the lobes provides a catalytic Asp
residue, positioned within the hallmark motif Asp-Thr/Set-Gly, to the active
site. Eukaryotic APs form peptidase family A1 of clan AA [E1]. Currently known
eukaryotic APs are:
Vertebrate gastric pepsins A and C (also known as gastricsin).
Vertebrate chymosin (rennin), involved in digestion and used for making
Yeast saccharopepsin (EC 18.104.22.168) (proteinase A) (gene PEP4). PEP4 is
implicated in posttranslational regulation of vacuolar hydrolases.
Yeast barrierpepsin (EC 22.214.171.124) (gene BAR1); a protease that cleaves
α-factor and thus acts as an antagonist of the mating pheromone.
Fission yeast sxa1 which is involved in degrading or processing the mating
Most retroviruses and some plant viruses, such as badnaviruses, encode for APs
which are β homodimers (see <PDB:1A30>), where the aspartates are located
on two loops at the monomer interface and where two β-hairpins cover the
active site. In most retroviruses, the protease is encoded as a segment of a
polyprotein which is cleaved during the maturation process of the virus. It is
generally part of the pol polyprotein and, more rarely, of the gag
polyprotein. Retroviral APs form peptidase family A2 of clan AA [E2].
Despite the structural differences and the low sequence identity, it is
believed that the eukaryotic and retroviral protease families are
evolutionarily related since, in both folds, the cleavage site loops are
homologous, the Asp dyad is located at an interface region, and the viral
subunits are structurally similar to the N-terminal lobes of the eukaryotic
family enzymes. Conservation of the sequence around the two aspartates of
eukaryotic APs and around the single active site of the viral proteases allows
us to develop a single signature pattern for both groups of protease. A
profile was developed to specifically detect viral aspartyl proteases, which
are missed by the pattern. Another profile is directed against the eukaryotic
peptidase family A1 catalytic domain.
These proteins belong to families A1 and A2 in the classification of
July 2015 / Text revised; profile added.
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Gastric proteinases--structure, function, evolution and mechanism of action.
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