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| PROSITE documentation PDOC00159 |
Eukaryotic DNA topoisomerase I active site
Description:
DNA topoisomerase I (EC 5.99.1.2) [1,2,3,4] is one of the two types of enzyme
that catalyze the interconversion of topological DNA isomers. Type I
topoisomerases act by catalyzing the transient breakage of DNA, one strand at
a time, and the subsequent rejoining of the strands. When a eukaryotic type 1
topoisomerase breaks a DNA backbone bond, it simultaneously forms a protein-DNA link where the hydroxyl group of a tyrosine residue is joined to a 3'-phosphate on DNA, at one end of the enzyme-severed DNA strand. In eukaryotes
and poxvirus topoisomerases I, there are a number of conserved residues in the
region around the active site tyrosine.
Last update:
December 2001 / Text revised.
Technical section:
PROSITE method (with tools and information) covered by this documentation:
| TOPOISOMERASE_I_EUK, PS00176; Eukaryotic DNA topoisomerase I active site (PATTERN) |
| Consensus pattern: |
[DEN]-x(6)-[GS]-[IT]-S-K-x(2)-Y-[LIVM]-x(3)-[LIVM]
Y is the active site tyrosine |
| Sequences known to belong to this class detected by the pattern: |
ALL |
| Other sequence(s) detected in Swiss-Prot: |
2 |
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| Matching PDB structures:
1A41 2H7G 3IGC [ALL] |
References:
| 1 |
Authors | Sternglanz R. |
| Title | DNA topoisomerases. |
| Source | Curr. Opin. Cell Biol. 1:533-535(1989). |
| PubMed ID | 2560656 |
| 2 |
Authors | Sharma A., Mondragon A. |
| Title | DNA topoisomerases. |
| Source | Curr. Opin. Struct. Biol. 5:39-47(1995). |
| PubMed ID | 7773745 |
| 3 |
Authors | Lynn R.M., Bjornsti M.-A., Caron P.R., Wang J.C. |
| Title | Peptide sequencing and site-directed mutagenesis identify tyrosine-727 as the active site tyrosine of Saccharomyces cerevisiae DNA topoisomerase I. |
| Source | Proc. Natl. Acad. Sci. U.S.A. 86:3559-3563(1989). |
| PubMed ID | 2542938 |
| 4 |
Authors | Roca J. |
| Title | The mechanisms of DNA topoisomerases. |
| Source | Trends Biochem. Sci. 20:156-160(1995). |
| PubMed ID | 7770916 |
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