|PROSITE documentation PDOC00317|
Ribonucleotide reductase (EC 18.104.22.168) [1,2] catalyzes the reductive synthesis of deoxyribonucleotides from their corresponding ribonucleotides. It provides the precursors necessary for DNA synthesis. Ribonucleotide reductase is an oligomeric enzyme composed of a large subunit (700 to 1000 residues) and a small subunit (300 to 400 residues).
The small chain binds two iron atoms  (three Glu, one Asp, and two His are involved in metal binding) and contains an active site tyrosine radical. The regions of the sequence that contain the metal-binding residues and the active site tyrosine are conserved in ribonucleotide reductase small chain from prokaryotes, eukaryotes and viruses.
We have selected one of these regions as a signature pattern. It contains the active site residue as well as a glutamate and a histidine involved in the binding of iron.Last update:
December 2001 / Pattern and text revised.
PROSITE method (with tools and information) covered by this documentation:
|1||Authors||Nillson O., Lundqvist T., Hahne S., Sjoberg B.-M.|
|Source||Biochem. Soc. Trans. 16:91-94(1988).|
|Title||From RNA to DNA, why so many ribonucleotide reductases?|
|3||Authors||Nordlund P., Sjoberg B.-M., Eklund H.|
|Title||Three-dimensional structure of the free radical protein of ribonucleotide reductase.|