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PROSITE documentation PDOC00321

CDP-alcohol phosphatidyltransferases signature

Description:

A number of phosphatidyltransferases, which are all involved in phospholipid biosynthesis and that share the property of catalyzing the displacement of CMP from a CDP-alcohol by a second alcohol with formation of a phosphodiester bond and concomitant breaking of a phosphoride anhydride bond share a conserved sequence region [1,2]. These enzymes are:

  • Ethanolaminephosphotransferase (EC 2.7.8.1) from yeast (gene EPT1).
  • Diacylglycerol cholinephosphotransferase (EC 2.7.8.2) from yeast (gene CPT1).
  • Phosphatidylglycerophosphate synthase (EC 2.7.8.5) (CDP-diacylglycerol-- glycerol-3-phosphate 3-phosphatidyltransferase) from bacteria (gene pgsA).
  • Phosphatidylserine synthase (EC 2.7.8.8) (CDP-diacylglycerol--serine O- phosphatidyltransferase) from yeast (gene CHO1) and from Bacillus subtilis (gene pssA).
  • Phosphatidylinositol synthase (EC 2.7.8.11) (CDP-diacylglycerol--inositol 3-phosphatidyltransferase) from yeast (gene PIS).

These enzymes are proteins of from 200 to 400 amino acid residues. The conserved region contains three aspartic acid residues and is located in the N-terminal section of the sequences.

Last update:

December 2004 / Pattern and text revised.

Technical section:

PROSITE method (with tools and information) covered by this documentation:

CDP_ALCOHOL_P_TRANSF, PS00379; CDP-alcohol phosphatidyltransferases signature  (PATTERN)
Consensus pattern: D-G-x(2)-A-R-x(7,8)-G-x(3)-D-x(3)-D
Sequences known to belong to this class detected by the pattern: ALL
Other sequence(s) detected in Swiss-Prot: NONE
• Retrieve an alignment of Swiss-Prot true positive hits:
  Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS00379
• Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS00379
• Scan Swiss-Prot/TrEMBL entries against PS00379
• view ligand binding statistics

References:

1 AuthorsNikawa J.-I., Kodaki T., Yamashita S.
TitlePrimary structure and disruption of the phosphatidylinositol synthase gene of Saccharomyces cerevisiae.
SourceJ. Biol. Chem. 262:4876-4881(1987).
PubMed ID3031032
2 AuthorsHjelmstad R.H., Bell R.M.
Titlesn-1,2-diacylglycerol choline- and ethanolaminephosphotransferases in Saccharomyces cerevisiae. Nucleotide sequence of the EPT1 gene and comparison of the CPT1 and EPT1 gene products.
SourceJ. Biol. Chem. 266:5094-5103(1991).
PubMed ID1848238

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