 |
|
| PROSITE documentation PDOC00326 |
Proteasome A-type subunit signature and profile
Description:
The proteasome (or macropain) (EC 3.4.25.1) [1,2,3,4,5,E1] is an eukaryotic and
archaebacterial multicatalytic proteinase complex that seems to be involved in
an ATP/ubiquitin-dependent nonlysosomal proteolytic pathway. In eukaryotes the
proteasome is composed of about 28 distinct subunits which form a highly
ordered ring-shaped structure (20S ring) of about 700 Kd.
Most proteasome subunits can be classified, on the basis on sequence
similarities into two groups, A and B. Subunits that belong to the A-type
group are proteins of from 210 to 290 amino acids that share a number of
conserved sequence regions. Subunits that are known to belong to this family
are listed below.
- Vertebrate subunits C2 (nu), C3, C8, C9, iota and zeta.
- Drosophila PROS-25, PROS-28.1, PROS-29 and PROS-35.
- Yeast C1 (PRS1), C5 (PRS3), C7-α (Y8) (PRS2), Y7, Y13, PRE5, PRE6 and
PUP2.
- Arabidopsis thaliana subunits α and PSM30.
- Thermoplasma acidophilum α-subunit. In this archaebacteria the
proteasome is composed of only two different subunits.
As a signature pattern for proteasome A-type subunits we selected the best
conserved region, which is located in the N-terminal part of these proteins.
We also have developed a profile which covers the whole conserved region.
Note:
These proteins belong to family T1 in the classification of peptidases
[6,E2].
Last update:
January 2010 / profile added and text revised.
Technical section:
PROSITE methods (with tools and information) covered by this documentation:
| PROTEASOME_A_2, PS51475; Proteasome A-type subunit profile (MATRIX) |
| Sequences known to belong to this class detected by the profile: |
ALL |
| Other sequence(s) detected in Swiss-Prot: |
NONE. |
|
|
|
| Matching PDB structures:
1FNT 1G0U 1G65 1IRU ... [ALL] |
| PROTEASOME_A_1, PS00388; Proteasome A-type subunits signature (PATTERN) |
| Consensus pattern: |
[FYNAGS]-x(4)-[STNLV]-x-[FYW]-S-[PDS]-x(0,1)-G-[RKHDS]-x(2)-Q-[LIVA]-[DENR]-[YH]-[GSAD]-x(2)-[GSAG]
|
| Sequences known to belong to this class detected by the pattern: |
ALL |
| Other sequence(s) detected in Swiss-Prot: |
NONE. |
|
|
|
| Matching PDB structures:
1FNT 1G0U 1G65 1IRU ... [ALL] |
References:
| 1 |
Authors | Rivett A.J. |
| Title | Proteasomes: multicatalytic proteinase complexes. |
| Source | Biochem. J. 291:1-10(1993). |
| PubMed ID | 7682410 |
| 2 |
Authors | Rivett A.J. |
| Title | The multicatalytic proteinase of mammalian cells. |
| Source | Arch. Biochem. Biophys. 268:1-8(1989). |
| PubMed ID | 2643381 |
| 3 |
Authors | Goldberg A.L., Rock K.L. |
| Title | Proteolysis, proteasomes and antigen presentation. |
| Source | Nature 357:375-379(1992). |
| PubMed ID | 1317508 |
| DOI | 10.1038/357375a0 |
| 4 |
Authors | Wilk S. |
| Title | Proteasomes. Multicatalytic proteinase complexes. |
| Source | Enzyme Protein 47:187-188(1993). |
| PubMed ID | 7697118 |
| 5 |
Authors | Hilt W., Wolf D.H. |
| Title | Proteasomes: destruction as a programme. |
| Source | Trends Biochem. Sci. 21:96-102(1996). |
| PubMed ID | 8882582 |
| 6 |
Authors | Rawlings N.D., Barrett A.J. |
| Title | Families of serine peptidases. |
| Source | Methods Enzymol. 244:19-61(1994). |
| PubMed ID | 7845208 |
Copyright:
PROSITE is copyright. It is produced by the Swiss Institute of
Bioinformatics (SIB). There are no restrictions on its use by non-profit
institutions as long as its content is in no way modified. Usage by and
for commercial entities requires a license agreement. For information
about the licensing scheme send an email to license@isb-sib.ch or
see: http://www.expasy.org/prosite/prosite_license.htm.
Miscellaneous:
View entry in original PROSITE document format
View entry in raw text format (no links)