{PDOC00357}
{PS00384; PROKAR_ZN_DEPEND_PLPC_1}
{PS51346; PROKAR_ZN_DEPEND_PLPC_2}
{BEGIN}
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* Prokaryotic zinc-dependent phospholipase C domain signature and profile *
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Bacillus cereus expresses  a phosphatidylcholine  hydrolyzing  phospholipase C
(EC 3.1.4.3) (PLC) which  is a  monomeric  protein  of 245 amino-acid residues
that binds three zinc ions [1].  This  PLC is  highly similar to the following
proteins:

 - Alpha-toxin from Clostridium  perfringens and bifermentans  which  are also
   zinc-dependent phospholipases C [2].
 - Lecithinase C from Listeria monocytogenes [3].

In Bacillus  cereus,  there  are nine residues known to be involved in binding
the zinc ions: 5 His, 2 Asp, 1 Glu and 1 Trp. These residues are all conserved
in the Clostridium alpha-toxin [4].

As  a  signature  pattern  for this family of enzymes, we selected a conserved
region  of  11  residues  that contains three of the zinc ligands: a histidine
involved  in binding the first zinc ion, an aspartic acid which binds both the
first and the third zinc ion, and a histidine which binds the second zinc ion.
We  also developed a profile that covers the entire prokaryotic zinc-dependent
phospholipase C domain.

-Consensus pattern: H-Y-x-[GT]-D-[LIVMAF]-[DNSH]-x-P-x-H-[PA]-x-N
                    [The 2 H's and the D bind zinc]
-Sequences known to belong to this class detected by the pattern: ALL.
-Other sequence(s) detected in Swiss-Prot: NONE.

-Sequences known to belong to this class detected by the profile: ALL.
-Other sequence(s) detected in Swiss-Prot: NONE.

-Last update: December 2007 / Text revised; profile added.

[ 1] Hough E., Hansen L.K., Birknes B., Jynge K., Hansen S., Hordvik A.,
     Little C., Dodson E., Derewenda Z.
     "High-resolution (1.5 A) crystal structure of phospholipase C from
     Bacillus cereus."
     Nature 338:357-360(1989).
     PubMed=2493587; DOI=10.1038/338357a0
[ 2] Titball R.W., Rubidge T.
     "The role of histidine residues in the alpha toxin of Clostridium
     perfringens."
     FEMS Microbiol. Lett. 56:261-265(1990).
     PubMed=2111259
[ 3] Vazquez-Boland J.A., Dominguez L., Rodriguez-Ferri E.F.,
     Fernandez-Garayzabal J.F., Suarez G.
     "Preliminary evidence that different domains are involved in cytolytic
     activity and receptor (cholesterol) binding in listeriolysin O, the
     Listeria monocytogenes thiol-activated toxin."
     FEMS Microbiol. Lett. 53:95-99(1989).
     PubMed=2515103
[ 4] Naylor C.E., Eaton J.T., Howells A., Justin N., Moss D.S.,
     Titball R.W., Basak A.K.
     "Structure of the key toxin in gas gangrene."
     Nat. Struct. Biol. 5:738-746(1998).
     PubMed=9699639; DOI=10.1038/1447

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