Home ScanProsite ProRule Documents Downloads Links Funding
PROSITE documentation PDOC00369

S-adenosylmethionine synthase signatures

Description:

S-adenosylmethionine synthase (EC 2.5.1.6) is the enzyme that catalyzes the formation of S-adenosylmethionine (AdoMet) from methionine and ATP [1]. AdoMet is an important methyl donor for transmethylation and is also the propylamino donor in polyamine biosynthesis.

In bacteria there is a single isoform of AdoMet synthase (gene metK), there are two in budding yeast (genes SAM1 and SAM2) and in mammals while in plants there is generally a multigene family.

The sequence of AdoMet synthase is highly conserved throughout isozymes and species. We selected two signature patterns for this type of enzyme; the first is a hexapeptide which seems to be involved in ATP-binding; the second is an almost perfectly conserved glycine-rich nonapeptide.

Last update:

April 2006 / Pattern revised.

Technical section:

PROSITE methods (with tools and information) covered by this documentation:

ADOMET_SYNTHASE_1, PS00376; S-adenosylmethionine synthase signature 1  (PATTERN)
Consensus pattern: [GN]-[AS]-G-D-Q-G-x(3)-G-[FYHG]
Sequences known to belong to this class detected by the pattern: ALL, except for M.genitalium metK
Other sequence(s) detected in Swiss-Prot: NONE.
• Retrieve an alignment of Swiss-Prot true positive hits:
  Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS00376
• Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS00376
• Scan Swiss-Prot/TrEMBL entries against PS00376
• view ligand binding statistics
Matching PDB structures: 1FUG 1MXA 1MXB 1MXC ... [ALL]
ADOMET_SYNTHASE_2, PS00377; S-adenosylmethionine synthase signature 2  (PATTERN)
Consensus pattern: G-[GA]-G-[ASC]-[FY]-S-x-K-[DE]
Sequences known to belong to this class detected by the pattern: ALL
Other sequence(s) detected in Swiss-Prot: NONE.
• Retrieve an alignment of Swiss-Prot true positive hits:
  Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS00377
• Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS00377
• Scan Swiss-Prot/TrEMBL entries against PS00377
• view ligand binding statistics
Matching PDB structures: 1FUG 1MXA 1MXB 1MXC ... [ALL]

Reference:

1 AuthorsHorikawa S., Sasuga J., Shimizu K., Ozasa H., Tsukada K.
TitleMolecular cloning and nucleotide sequence of cDNA encoding the rat kidney S-adenosylmethionine synthetase.
SourceJ. Biol. Chem. 265:13683-13686(1990).
PubMed ID1696256

Copyright:

PROSITE is copyright. It is produced by the Swiss Institute of Bioinformatics (SIB). There are no restrictions on its use by non-profit institutions as long as its content is in no way modified. Usage by and for commercial entities requires a license agreement. For information about the licensing scheme send an email to license@isb-sib.ch or see: http://www.expasy.org/prosite/prosite_license.htm.

Miscellaneous:

View entry in original PROSITE document format
View entry in raw text format (no links)