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| PROSITE documentation PDOC00410 |
Bacteriophage-type RNA polymerase family active site signatures
Description:
Many forms of RNA polymerase (EC 2.7.7.6) are known. Most RNA polymerases are
multimeric enzymes, but there is a family of single chain polymerases, which
are evolutionary related, and which originate from bacteriophages or from
mitochondria. The RNA polymerases that belong to this family are [1]:
- Podoviridae bacteriophages T3, T7, and K11 polymerase.
- Bacteriophage SP6 polymerase.
- Vertebrate mitochondrial polymerase (gene POLRMT).
- Fungal mitochondrial polymerase (gene RPO41).
- Polymerases encoded on mitochondrial linear DNA plasmids in various fungi
and plants: Agaricus bitorquis pEM, Claviceps purpurea pClK1, Neurospora
crassa Kalilo; Neurospora intermedia Maranhar and maize S-2).
Two conserved aspartate and one lysine residue have been shown [2,3] to be
part of the active site of T7 polymerase. We have used the regions around the
first aspartate and around the lysine as signature patterns for this family of
polymerases.
Last update:
July 1999 / Text revised.
Technical section:
PROSITE methods (with tools and information) covered by this documentation:
| RNA_POL_PHAGE_1, PS00900; Bacteriophage-type RNA polymerase family active site signature 1 (PATTERN) |
| Consensus pattern: |
P-[LIVM]-x(2)-D-[GA]-[ST]-[AC]-[SN]-[GA]-[LIVMFY]-Q
D is the active site residue |
| Sequences known to belong to this class detected by the pattern: |
ALL |
| Other sequence(s) detected in Swiss-Prot: |
NONE. |
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| Matching PDB structures:
1ARO 1CEZ 1H38 1MSW ... [ALL] |
| RNA_POL_PHAGE_2, PS00489; Bacteriophage-type RNA polymerase family active site signature 2 (PATTERN) |
| Consensus pattern: |
[LIVMF]-x-R-x(3)-K-x(2)-[LIVMF]-M-[PT]-x(2)-Y
K is the active site residue |
| Sequences known to belong to this class detected by the pattern: |
ALL |
| Other sequence(s) detected in Swiss-Prot: |
NONE. |
|
|
|
| Matching PDB structures:
1ARO 1CEZ 1H38 1MSW ... [ALL] |
References:
| 1 |
Authors | McAllister W.T., Raskin C.A. |
| Title | The phage RNA polymerases are related to DNA polymerases and reverse transcriptases. |
| Source | Mol. Microbiol. 10:1-6(1993). |
| PubMed ID | 7526118 |
| 2 |
Authors | Maksimova T.G., Mustayev A.A., Zaychikov E.F., Lyakhov D.L., Tunitskaya V.L., Akbarov A.K., Luchin S.V., Rechinsky V.O., Chernov B.K., Kochetkov S.N. |
| Title | Lys631 residue in the active site of the bacteriophage T7 RNA polymerase. Affinity labeling and site-directed mutagenesis. |
| Source | Eur. J. Biochem. 195:841-847(1991). |
| PubMed ID | 1847871 |
| 3 |
Authors | Sousa R., Chung Y.J., Rose J.P., Wang B.-C. |
| Title | Crystal structure of bacteriophage T7 RNA polymerase at 3.3 A resolution. |
| Source | Nature 364:593-599(1993). |
| PubMed ID | 7688864 |
| DOI | 10.1038/364593a0 |
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