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PROSITE documentation PDOC00444

Adenylosuccinate synthetase signatures

Description:

Adenylosuccinate synthetase (EC 6.3.4.4) [1] plays an important role in purine biosynthesis, by catalyzing the GTP-dependent conversion of IMP and aspartic acid to AMP. Adenylosuccinate synthetase has been characterized from various sources ranging from Escherichia coli (gene purA) to vertebrate tissues. In vertebrates, two isozymes are present - one involved in purine biosynthesis and the other in the purine nucleotide cycle.

As signature patterns, we selected two conserved regions. The first one is a perfectly conserved octapeptide located in the N-terminal section and which is involved in GTP-binding [2]. The second one includes a lysine residue known [2] to be essential for the enzyme's activity.

Last update:

December 2004 / Pattern and text revised.

Technical section:

PROSITE methods (with tools and information) covered by this documentation:

ADENYLOSUCCIN_SYN_1, PS01266; Adenylosuccinate synthetase GTP-binding site  (PATTERN)
Consensus pattern: [QGF]-[WLCF]-G-D-E-[GA]-K-[GA]
Sequences known to belong to this class detected by the pattern: ALL
Other sequence(s) detected in Swiss-Prot: NONE.
• Retrieve an alignment of Swiss-Prot true positive hits:
  Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS01266
• Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS01266
• Scan Swiss-Prot/TrEMBL entries against PS01266
• view ligand binding statistics
Matching PDB structures: 1ADE 1ADI 1CG0 1CG3 ... [ALL]
ADENYLOSUCCIN_SYN_2, PS00513; Adenylosuccinate synthetase active site  (PATTERN)
Consensus pattern: G-I-[GR]-P-x-Y-x(2)-K-x(2)-R
K is the active site residue
Sequences known to belong to this class detected by the pattern: ALL, except for archebacterial purA [3]
Other sequence(s) detected in Swiss-Prot: NONE.
• Retrieve an alignment of Swiss-Prot true positive hits:
  Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS00513
• Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS00513
• Scan Swiss-Prot/TrEMBL entries against PS00513
• view ligand binding statistics
Matching PDB structures: 1ADE 1ADI 1CG0 1CG1 ... [ALL]

References:

1 AuthorsWiesmueller L., Wittbrodt J., Noegel A.A., Schleicher M.
SourceJ. Biol. Chem. 266:2480-2485(1991).
2 AuthorsSilva M.M., Poland B.W., Hoffman C.R., Fromm H.J., Honzatko R.B.
TitleRefined crystal structures of unligated adenylosuccinate synthetase from Escherichia coli.
SourceJ. Mol. Biol. 254:431-446(1995).
PubMed ID7490761
3 AuthorsBouyoub A., Barbier G., Forterre P., Labedan B.
TitleThe adenylosuccinate synthetase from the hyperthermophilic archaeon Pyrococcus species displays unusual structural features.
SourceJ. Mol. Biol. 261:144-154(1996).
PubMed ID8757283

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