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PROSITE documentation PDOC00493

Bacterial ring hydroxylating dioxygenases alpha-subunit signature

Description:

Bacterial ring hydroxylating dioxygenases are multicomponent enzymatic complexes composed of a hydroxylase component and an electron transfer component. The hydroxylase component is itself composed of two subunits: an α-subunit of about 50 Kd, and a β-subunit of about 20 Kd. The electron transfer component is either composed of two subunits: a ferredoxin and a ferredoxin reductase or by a single bifunctional ferredoxin/reductase subunit.

The α-subunit of hydroxylase components are evolutionary related [1]. The currently known members of this family are listed below.

  • Benzene 1,2-dioxygenase (EC 1.14.12.3) (gene bnzA) from Pseudomonas putida.
  • phthalate 4,5-dioxygenase (EC 1.14.12.7) (gene pht3) from Pseudomonas.
  • Benzoate 1,2-dioxygenase (EC 1.14.12.10) (gene benA) from Acinetobacter calcoaceticus.
  • Naphthalene 1,2-dioxygenase (EC 1.14.12.12) (gene ndoC) from Pseudomonas.
  • 3-chlorobenzoate-3,4-dioxygenase (EC 1.14.12-.-) (gene cbaA) from Alcaligenes.
  • Biphenyl dioxygenase (EC 1.14.99.-) (gene bphA) from Pseudomonas.
  • Toluate 1,2-dioxygenase (EC 1.14.12.-) (gene xylX) from Pseudomonas putida.
  • Toluene 2,3-dioxygenase (EC 1.14.12.-) (gene todC1) from Pseudomonas putida.

The α-subunit of the hydroxylase components bind both a 2Fe-2S type iron-sulfur center and an iron atom. There is, in the N-terminal section of these proteins, a conserved region of 24 residues which contains two cysteines and two histidines which have been shown [2] to be involved in the binding of the iron-sulfur center. We have used this region has a signature pattern.

Expert(s) to contact by email:

Harayama S.

Last update:

December 2004 / Pattern and text revised.

Technical section:

PROSITE method (with tools and information) covered by this documentation:

RING_HYDROXYL_ALPHA, PS00570; Bacterial ring hydroxylating dioxygenases alpha-subunit signature  (PATTERN)
Consensus pattern: C-x-H-R-[GAR]-x(7,8)-[GEKVI]-[NERAQ]-x(4,5)-C-x-[FY]-H
The 2 C's and the 2 H's are 2Fe-2S ligands
Sequences known to belong to this class detected by the pattern: ALL
Other sequence(s) detected in Swiss-Prot: NONE.
• Retrieve an alignment of Swiss-Prot true positive hits:
  Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS00570
• Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS00570
• Scan Swiss-Prot/TrEMBL entries against PS00570
• view ligand binding statistics
Matching PDB structures: 1EG9 1NDO 1O7G 1O7H ... [ALL]

References:

1 AuthorsNeidle E.L., Hartnett C., Ornston L.N., Bairoch A., Rekik M., Harayama S.
TitleNucleotide sequences of the Acinetobacter calcoaceticus benABC genes for benzoate 1,2-dioxygenase reveal evolutionary relationships among multicomponent oxygenases.
SourceJ. Bacteriol. 173:5385-5395(1991).
PubMed ID1885518
2 AuthorsKauppi B., Lee K., Carredano E., Parales R.E., Gibson D.T., Eklund H., Ramaswamy S.
TitleStructure of an aromatic-ring-hydroxylating dioxygenase-naphthalene 1,2-dioxygenase.
SourceStructure 6:571-586(1998).
PubMed ID9634695

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