It has been shown [1,2,3] that several enzymes from various prokaryotic and
eukaryotic organisms which are involved in the hydrolysis of amides (amidases)
are evolutionary related. These enzymes are listed below.
Indoleacetamide hydrolase (EC 3.5.1.-), a bacterial plasmid-encoded enzyme
that catalyzes the hydrolysis of indole-3-acetamide (IAM) into indole-3-
acetate (IAA), the second step in the biosynthesis of auxins from
Acetamidase from Emericella nidulans (gene amdS), an enzyme which allows
acetamide to be used as a sole carbon or nitrogen source.
Amidase (EC 22.214.171.124) from Rhodococcus sp. N-774 and Brevibacterium sp. R312
(gene amdA). This enzyme hydrolyzes propionamides efficiently, and also at
a lower efficiency, acetamide, acrylamide and indoleacetamide.
Amidase (EC 126.96.36.199) from Pseudomonas chlororaphis.
6-aminohexanoate-cyclic-dimer hydrolase (EC 188.8.131.52) (nylon oligomers
degrading enzyme E1) (gene nylA), a bacterial plasmid encoded enzyme which
catalyzes the first step in the degradation of 6-aminohexanoic acid cyclic
dimer, a by-product of nylon manufacture .
Glutamyl-tRNA(Gln) amidotransferase subunit A .
PROSITE is copyright. It is produced by the SIB Swiss Institute
Bioinformatics. There are no restrictions on its use by non-profit
institutions as long as its content is in no way modified. Usage by and
for commercial entities requires a license agreement. For information
about the licensing scheme send an email to
or see: prosite_license.html.