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It has been shown [1,2] that the following glycosyl hydrolases can be classified into a single family on the basis of sequence similarities:

• Glucan endo-1,3-β-D-glucosidases (EC 3.2.1.39) (endo-(1->3)-β- glucanase) from various plants. This enzyme may be involved in the defense of plants against pathogens through its ability to degrade fungal cell wall polysaccharides.
• Glucan 1,3-β-glucosidase (EC 3.2.1.58) (exo-(1->3)-β-glucanase) from yeast (gene BGL2). This enzyme may play a role in cell expansion during growth, in cell-cell fusion during mating, and in spore release during sporulation.
• Lichenases (EC 3.2.1.73) (endo-(1->3,1->4)-β-glucanase) from various plants.

The best conserved region in the sequence of these enzymes is located in their central section. This region contains a conserved tryptophan residue which could be involved in the interaction with the glucan substrates [2] and it also contains a conserved glutamate which has been shown [3] to act as the nucleophile in the catalytic mechanism. We have used this region as a signature pattern.

Henrissat B.

December 2004 / Pattern and text revised.

PROSITE method (with tools and information) covered by this documentation:

GLYCOSYL_HYDROL_F17, PS00587; Glycosyl hydrolases family 17 signature  (PATTERN)

Consensus pattern: [LIVMKS]-x-[LIVMFYWA](3)-[STAG]-E-[STACVI]-G-[WY]-P-[STN]-x-[SAGQ] E is an active site residue Sequences known to belong to this class detected by the pattern: ALL Other sequence(s) detected in Swiss-Prot: NONE.

• Retrieve an alignment of Swiss-Prot true positive hits:   Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format • Retrieve the sequence logo from the alignment • Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS00587 • Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS00587 • Scan Swiss-Prot/TrEMBL entries against PS00587 • view ligand binding statistics

Matching PDB structures: 1AQ0 1GHR 1GHS [ALL]

1	Authors	Henrissat B.
	Title	A classification of glycosyl hydrolases based on amino acid sequence similarities.
	Source	Biochem. J. 280:309-316(1991).
	PubMed ID	1747104

2	Authors	Ori N., Sessa G., Lotan T., Himmelhoch S., Fluhr R.
	Title	A major stylar matrix polypeptide (sp41) is a member of the pathogenesis-related proteins superclass.
	Source	EMBO J. 9:3429-3436(1990).
	PubMed ID	2120041

3	Authors	Varghese J.N., Garrett T.P.J., Colman P.M., Chen L., Hoj P.B., Fincher G.B.
	Title	Three-dimensional structures of two plant beta-glucan endohydrolases with distinct substrate specificities.
	Source	Proc. Natl. Acad. Sci. U.S.A. 91:2785-2789(1994).
	PubMed ID	8146192

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