|PROSITE documentation PDOC00518
Aminotransferases class-II pyridoxal-phosphate attachment site
Aminotransferases share certain mechanistic features with other pyridoxal-phosphate dependent enzymes, such as the covalent binding of the pyridoxal-phosphate group to a lysine residue. On the basis of sequence similarity,
these various enzymes can be grouped  into subfamilies. One of these,
called class-II, currently consists of the following enzymes:
- Glycine C-acetyltransferase (EC 18.104.22.168), which catalyzes the addition of
acetyl-CoA to glycine to form 2-amino-3-oxobutanoate (gene kbl).
- 5-aminolevulinic acid synthase (EC 22.214.171.124) (delta-ALA synthase), which
catalyzes the first step in heme biosynthesis via the Shemin (or C4)
pathway, i.e. the addition of succinyl-CoA to glycine to form 5-
- 8-amino-7-oxononanoate synthase (EC 126.96.36.199) (7-KAP synthase), a bacterial
enzyme (gene bioF) which catalyzes an intermediate step in the biosynthesis
of biotin: the addition of 6-carboxy-hexanoyl-CoA to alanine to form 8-
- Histidinol-phosphate aminotransferase (EC 188.8.131.52), which catalyzes the
eighth step in histidine biosynthetic pathway: the transfer of an amino
group from 3-(imidazol-4-yl)-2-oxopropyl phosphate to glutamic acid to form
histidinol phosphate and 2-oxoglutarate.
- Serine C-palmitoyltransferase (EC 184.108.40.206) from yeast (genes LCB1 and
LCB2), which catalyzes the condensation of palmitoyl-CoA and serine to form
The sequence around the pyridoxal-phosphate attachment site of this class of
enzyme is sufficiently conserved to allow the creation of a specific pattern.
December 2004 / Pattern and text revised.
PROSITE method (with tools and information) covered by this documentation:
|AA_TRANSFER_CLASS_2, PS00599; Aminotransferases class-II pyridoxal-phosphate attachment site (PATTERN)
K is the pyridoxal-P attachment site
|Sequences in UniProtKB/Swiss-Prot known to belong to this class detected by PS00599:
||653 true positives with 316 false negatives and 2 partials.
|Other sequence(s) in UniProtKB/Swiss-Prot detected by PS00599:
||74 false positives.
|Matching PDB structures:
1BS0 1DJ9 1DJE 1FC4 ... [ALL]
||Unpublished observations (1991).
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