PROSITE documentation PDOC00524

Thymidine kinase cellular-type signature

Description

Thymidine kinase (TK) (EC 2.7.1.21) is an ubiquitous enzyme that catalyzes the ATP-dependent phosphorylation of thymidine. A comparison of TK sequences has shown [1,2,3] that there are two different families of TK. One family groups together TK from herpesviruses as well as cellular thymidylate kinases, while the second family currently consists of TK from the following sources:

  • Vertebrates.
  • Bacterial.
  • Bacteriophage T4.
  • Poxviruses.
  • African swine fever virus (ASF).

As a signature pattern for this family of TK we selected a conserved region which is located in the C-terminal section of these enzymes.

Note:

Proteins belonging to this family also contain a copy of the ATP/GTP- binding motif 'A' (P-loop) in their N-terminal extremity (see <PDOC00017>).

Last update:

December 2004 / Pattern and text revised.

Technical section

PROSITE method (with tools and information) covered by this documentation:

TK_CELLULAR_TYPE, PS00603; Thymidine kinase cellular-type signature  (PATTERN)
Consensus pattern: [GA]-x(1,2)-[DE]-x-Y-x-[STAPV]-x-C-[NKR]-x-[CH]-[LIVMFYWH]
Sequences known to belong to this class detected by the pattern: ALL
Other sequence(s) detected in Swiss-Prot: NONE.
• Retrieve an alignment of Swiss-Prot true positive hits:
  Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS00603
• Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS00603
• Scan Swiss-Prot/TrEMBL entries against PS00603
• view ligand binding statistics
Matching PDB structures: 1W4R 1XBT 2B8T 2J87 ... [ALL]

References

1 Authors Boyle D.B., Coupar B.E.H., Gibbs A.J., Seigman L.J., Both G.W.
Title Fowlpox virus thymidine kinase: nucleotide sequence and relationships to other thymidine kinases.
Source Virology 156:355-365(1987).
PubMed ID 3027984
2 Authors Blasco R., Lopez-Otin C., Munoz M., Bockamp E.-O., Simon-Mateo C., Vinuela E.
Title Sequence and evolutionary relationships of African swine fever virus thymidine kinase.
Source Virology 178:301-304(1990).
PubMed ID 2389555
3 Authors Robertson G.R., Whalley J.M.
Title Evolution of the herpes thymidine kinase: identification and comparison of the equine herpesvirus 1 thymidine kinase gene reveals similarity to a cell-encoded thymidylate kinase.
Source Nucleic Acids Res. 16:11303-11317(1988).
PubMed ID 2849761

Copyright

PROSITE is copyright. It is produced by the SIB Swiss Institute Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified. Usage by and for commercial entities requires a license agreement. For information about the licensing scheme send an email to
Prosite License or see: prosite_license.html.

Miscellaneous

View entry in original PROSITE document format
View entry in raw text format (no links)