{PDOC00530} {PS00607; PDEASE_II} {BEGIN} ********************************************** * cAMP phosphodiesterases class-II signature * ********************************************** Cyclic AMP phosphodiesterase (EC 3.1.4.17) (cAMP-PDEase) catalyzes the hydrolysis of cAMP to the corresponding nucleoside 5' monophosphate. While most PDEases can be, on the basis of sequence similarities, grouped together [1], there are at least two enzymes which do not belong to the main family of PDEases and which represent a second class of these enzymes [2]: - Slime mold Dictyostelium discoideum extracellular cAMP PDEase. This enzyme plays an essential role in development by hydrolyzing the cAMP used as a chemoattractant by aggregating cells. - Budding yeast and Candida albicans low-affinity cAMP PDEase (gene PDE1). - Fission yeast probable cAMP PDEase (gene cgs2). - Vibrio fischeri periplasmic cAMP PDEase (gene cpdP) [3]. There is, in the central part of these enzymes, a highly conserved region which contains three histidines. We have used this region as a signature pattern. -Consensus pattern: H-x-H-L-D-H-[LIVM]-x-[GS]-[LIVMA]-[LIVM](2)-x-S-[AP] -Sequences known to belong to this class detected by the pattern: ALL. -Other sequence(s) detected in Swiss-Prot: NONE. -Last update: December 2001 / Text revised. [ 1] Beavo J.A., Reifsnyder D.H. "Primary sequence of cyclic nucleotide phosphodiesterase isozymes and the design of selective inhibitors." Trends Pharmacol. Sci. 11:150-155(1990). PubMed=2159198 [ 2] Nikawa J.-I., Sass P., Wigler M. "Cloning and characterization of the low-affinity cyclic AMP phosphodiesterase gene of Saccharomyces cerevisiae." Mol. Cell. Biol. 7:3629-3636(1987). PubMed=2824992 [ 3] Dunlap P.V., Callahan S.M. "Characterization of a periplasmic 3':5'-cyclic nucleotide phosphodiesterase gene, cpdP, from the marine symbiotic bacterium Vibrio fischeri." J. Bacteriol. 175:4615-4624(1993). PubMed=8393003 -------------------------------------------------------------------------------- PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see https://prosite.expasy.org/prosite_license.html -------------------------------------------------------------------------------- {END}