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| PROSITE documentation PDOC00554 |
Respiratory-chain NADH dehydrogenase 75 Kd subunit signatures
Description:
Respiratory-chain NADH dehydrogenase (EC 1.6.5.3) [1,2] (also known as complex
I or NADH-ubiquinone oxidoreductase) is an oligomeric enzymatic complex
located in the inner mitochondrial membrane which also seems to exist in
the chloroplast and in cyanobacteria (as a NADH-plastoquinone oxidoreductase).
Among the 25 to 30 polypeptide subunits of this bioenergetic enzyme complex
there is one with a molecular weight of 75 Kd (in mammals), which is the
largest subunit of complex I and is a component of the iron-sulfur (IP)
fragment of the enzyme. It seems to bind to two 4Fe-4S clusters (called N-3
and N-4).
The 75 Kd subunit is highly similar to [3,4]:
- Subunit γ of Alcaligenes eutrophus NAD-reducing hydrogenase (gene hoxU)
which also binds two 4Fe-4S cluster.
- Subunit NQO3 of Paracoccus denitrificans NADH-ubiquinone oxidoreductase.
- Subunit G of Escherichia coli NADH-ubiquinone oxidoreductase (gene nuoG).
The 75 Kd subunit and the bacterial hydrogenase γ subunit contains three
conserved clusters of cysteine residues which are most probably involved in
the binding of the iron-sulfur clusters. We have developed signature patterns
for these three regions.
Last update:
December 2004 / Pattern and text revised.
Technical section:
PROSITE methods (with tools and information) covered by this documentation:
| COMPLEX1_75K_1, PS00641; Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 1 (PATTERN) |
| Consensus pattern: |
P-x(2)-C-[YWSD]-x(7)-[GA]-x-C-R-x-C
The 3 C's may be 4Fe-4S ligands |
| Sequences known to belong to this class detected by the pattern: |
ALL |
| Other sequence(s) detected in Swiss-Prot: |
NONE. |
|
|
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| Matching PDB structures:
2FUG 2YBB 3I9V 3IAM ... [ALL] |
| COMPLEX1_75K_2, PS00642; Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 2 (PATTERN) |
| Consensus pattern: |
C-P-x-C-[DE]-x-[GS](2)-x-C-x-L-Q
The 3 C's may be 4Fe-4S ligands |
| Sequences known to belong to this class detected by the pattern: |
ALL |
| Other sequence(s) detected in Swiss-Prot: |
NONE. |
|
|
|
| Matching PDB structures:
2FUG 2YBB 3I9V 3IAM ... [ALL] |
| COMPLEX1_75K_3, PS00643; Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 3 (PATTERN) |
| Consensus pattern: |
R-C-[LIVM]-x-C-x-R-C-[LIVMT]-x-[LMFY]
The 3 C's may be 4Fe-4S ligands |
| Sequences known to belong to this class detected by the pattern: |
ALL, except for NQO3 and nuoG |
| Other sequence(s) detected in Swiss-Prot: |
NONE. |
|
|
|
| Matching PDB structures:
2FUG 2YBB 3I9V 3IAM ... [ALL] |
References:
| 1 |
Authors | Ragan C.I. |
| Source | Curr. Top. Bioenerg. 15:1-36(1987). |
| 2 |
Authors | Weiss H., Friedrich T., Hofhaus G., Preis D. |
| Title | The respiratory-chain NADH dehydrogenase (complex I) of mitochondria. |
| Source | Eur. J. Biochem. 197:563-576(1991). |
| PubMed ID | 2029890 |
| 3 |
Authors | Fearnley I.M., Walker J.E. |
| Title | Conservation of sequences of subunits of mitochondrial complex I and their relationships with other proteins. |
| Source | Biochim. Biophys. Acta 1140:105-134(1992). |
| PubMed ID | 1445936 |
| 4 |
Authors | Weidner U., Geier S., Ptock A., Friedrich T., Leif H., Weiss H. |
| Title | The gene locus of the proton-translocating NADH: ubiquinone oxidoreductase in Escherichia coli. Organization of the 14 genes and relationship between the derived proteins and subunits of mitochondrial complex I. |
| Source | J. Mol. Biol. 233:109-122(1993). |
| PubMed ID | 7690854 |
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