{PDOC00558}
{PS00648; RIBONUCLEASE_P}
{BEGIN}
********************************************************
* Bacterial ribonuclease P protein component signature *
********************************************************

Ribonuclease P (EC 3.1.26.5) (RNase P) [1,2,3] is a site specific endonuclease
that generates  mature tRNAs by  cleaving-off  the  leader sequences  at their
5'ends. In bacteria RNase P is known to be composed of two components: a large
(about 400 base pairs) RNA (gene rnpB) and  a small  protein (119 to 133 amino
acids) (gene rnpA).  The RNA moiety of RNase P carries the catalytic activity;
the function of the protein component  is not yet clear although it may act as
an electrostatic screen  allowing  the  highly  negatively charged RNA enzyme-
substrate complex to fold into the catalytic conformation.

The sequence of rnpA is not highly conserved, however there is, in the central
part of the  protein,  a conserved basic region. We have used this region as a
signature pattern.

-Consensus pattern: [LIVMFYSNAD]-x(2)-A-x(2)-R-[NH]-[KRQLYAT]-[LIVMFSA]-[KRA]-
                    R-x-[LIVMTA]-[KR]
-Sequences known to belong to this class detected by the pattern: ALL.
-Other sequence(s) detected in Swiss-Prot: 2.
-Last update: December 2004 / Pattern and text revised.

[ 1] Pace N.R., Smith D.
     "Ribonuclease P: function and variation."
     J. Biol. Chem. 265:3587-3590(1990).
     PubMed=1689306
[ 2] Altman S.
     "Ribonuclease P. Postscript."
     J. Biol. Chem. 265:20053-20056(1990).
     PubMed=1700778
[ 3] Brown J.W., Pace N.R.
     "Ribonuclease P RNA and protein subunits from bacteria."
     Nucleic Acids Res. 20:1451-1456(1992).
     PubMed=1374553

--------------------------------------------------------------------------------
PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and
distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives
(CC BY-NC-ND 4.0) License, see https://prosite.expasy.org/prosite_license.html
--------------------------------------------------------------------------------

{END}