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PROSITE documentation PDOC00586

Prokaryotic-type carbonic anhydrases signatures

Description:

Carbonic anhydrases (EC 4.2.1.1) (CA) are zinc metalloenzymes which catalyze the reversible hydration of carbon dioxide.

In Escherichia coli, CA (gene cynT) is involved in recycling carbon dioxide formed in the bicarbonate-dependent decomposition of cyanate by cyanase (gene cynS). By this action, it prevents the depletion of cellular bicarbonate [1]. In photosynthetic bacteria and plant chloroplast, CA is essential to inorganic carbon fixation [2].

Prokaryotic and plant chloroplast CA are structurally and evolutionary related and form a family distinct from the one which groups the many different forms of eukaryotic CA's (see <PDOC00146>).

Hypothetical proteins yadF from Escherichia coli and HI1301 from Haemophilus influenzae also belong to this family.

We developed two signature patterns for this family of enzymes. Both patterns contain conserved residues that could be involved in binding zinc (cysteine and histidine).

Last update:

December 2004 / Pattern and text revised.

Technical section:

PROSITE methods (with tools and information) covered by this documentation:

PROK_CO2_ANHYDRASE_1, PS00704; Prokaryotic-type carbonic anhydrases signature 1  (PATTERN)
Consensus pattern: C-[SA]-D-S-R-[LIVM]-x-[AP]
Sequences known to belong to this class detected by the pattern: ALL
Other sequence(s) detected in Swiss-Prot: 1.
• Retrieve an alignment of Swiss-Prot true positive hits:
  Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS00704
• Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS00704
• Scan Swiss-Prot/TrEMBL entries against PS00704
• view ligand binding statistics
Matching PDB structures: 1EKJ 1I6O 1I6P 1T75 ... [ALL]
PROK_CO2_ANHYDRASE_2, PS00705; Prokaryotic-type carbonic anhydrases signature 2  (PATTERN)
Consensus pattern: [EQ]-[YF]-A-[LIVM]-x(2)-[LIVM]-x(4)-[LIVMF](3)-x-G-H-x(2)-C-G
Sequences known to belong to this class detected by the pattern: ALL
Other sequence(s) detected in Swiss-Prot: NONE.
• Retrieve an alignment of Swiss-Prot true positive hits:
  Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS00705
• Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS00705
• Scan Swiss-Prot/TrEMBL entries against PS00705
• view ligand binding statistics
Matching PDB structures: 1EKJ 1I6O 1I6P 1T75 ... [ALL]

References:

1 AuthorsGuilloton M.B., Korte J.J., Lamblin A.F., Fuchs J.A., Anderson P.M.
TitleCarbonic anhydrase in Escherichia coli. A product of the cyn operon.
SourceJ. Biol. Chem. 267:3731-3734(1992).
PubMed ID1740425
2 AuthorsFukuzawa H., Suzuki E., Komukai Y., Miyachi S.
TitleA gene homologous to chloroplast carbonic anhydrase (icfA) is essential to photosynthetic carbon dioxide fixation by Synechococcus PCC7942.
SourceProc. Natl. Acad. Sci. U.S.A. 89:4437-4441(1992).
PubMed ID1584776

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