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PROSITE documentation PDOC00589

Phosphoglucomutase and phosphomannomutase phosphoserine signature

  • Phosphoglucomutase (EC (PGM). PGM is an enzyme responsible for the conversion of D-glucose 1-phosphate into D-glucose 6-phosphate. PGM participates in both the breakdown and synthesis of glucose [1].
  • Phosphomannomutase (EC (PMM). PMM is an enzyme responsible for the conversion of D-mannose 1-phosphate into D-mannose 6-phosphate. PMM is required for different biosynthetic pathways in bacteria. For example, in enterobacteria such as Escherichia coli there are two different genes coding for this enzyme: rfbK which is involved in the synthesis of the O antigen of lipopolysaccharide and cpsG which is required for the synthesis of the M antigen capsular polysaccharide [2]. In Pseudomonas aeruginosa PMM (gene algC) is involved in the biosynthesis of the alginate layer [3] and in Xanthomonas campestris (gene xanA) it is involved in the biosynthesis of xanthan [4]. In Rhizobium strain ngr234 (gene noeK) it is involved in the biosynthesis of the nod factor.
  • Phosphoacetylglucosamine mutase (EC which converts N-acetyl-D- glucosamine 1-phosphate into the 6-phosphate isomer.

The catalytic mechanism of both PGM and PMM involves the formation of a phosphoserine intermediate [1]. The sequence around the serine residue is well conserved and can be used as a signature pattern.

In addition to PGM and PMM there are at least three uncharacterized proteins that belong to this family [5,6]:

  • Urease operon protein ureC from Helicobacter pylori.
  • Escherichia coli protein mrsA.
  • Paramecium tetraurelia parafusin, a phosphoglycoprotein involved in exocytosis.
  • A Methanococcus vannielii hypothetical protein in the 3'region of the gene for ribosomal protein S10.

PMM from fungi do not belong to this family.

Last update:

December 2001 / Pattern and text revised.

Technical section

PROSITE method (with tools and information) covered by this documentation:

PGM_PMM, PS00710; Phosphoglucomutase and phosphomannomutase phosphoserine signature  (PATTERN)


1AuthorsDai J.B., Liu Y., Ray W.J. Jr., Konno M.
TitleThe crystal structure of muscle phosphoglucomutase refined at 2.7-angstrom resolution.
SourceJ. Biol. Chem. 267:6322-6337(1992).
PubMed ID1532581

2AuthorsStevenson G., Lee S.J., Romana L.K., Reeves P.R.
TitleThe cps gene cluster of Salmonella strain LT2 includes a second mannose pathway: sequence of two genes and relationship to genes in the rfb gene cluster.
SourceMol. Gen. Genet. 227:173-180(1991).
PubMed ID1712067

3AuthorsZielinski N.A., Chakrabarty A.M., Berry A.
TitleCharacterization and regulation of the Pseudomonas aeruginosa algC gene encoding phosphomannomutase.
SourceJ. Biol. Chem. 266:9754-9763(1991).
PubMed ID1903398

4AuthorsKoeplin R., Arnold W., Hoette B., Simon R., Wang G., Puehler A.
SourceJ. Bacteriol. 174:191-199(1992).

5AuthorsBairoch A.
SourceUnpublished observations (1993).

6AuthorsSubramanian S.V., Wyroba E., Andersen A.P., Satir B.H.
SourceProc. Natl. Acad. Sci. U.S.A. 91:9832-9836(1994).

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