PROSITE documentation PDOC00604

MAM domain signature and profile

Description

A domain of about 170 amino acids has been recognized [1] in the extracellular region of functionally diverse proteins. All these proteins have a modular, receptor-like architecture consisting of a signal peptide, followed by a large N-terminal extracellular domain, a single transmembrane region and a intracellular domain. These proteins are listed below.

  • Meprin. This cell surface glycoprotein contains a zinc-metalloprotease domain capable of degrading a variety of polypeptides. Meprin is composed of two structurally related subunits (α and β) that form homo- or heterotetramers by the non-covalent association of two disulfide-linked dimers. In both subunits, the MAM domain is located after the catalytic domain.
  • Neuropilin (A5 antigen), a calcium-independent cell adhesion molecule that function during the formation of certain neuronal circuits. The sequence contains 2 CUB domains (see <PDOC00908>, 2 FA58C domains (see <PDOC00988>) and a MAM domain.
  • Receptor-like tyrosine protein phosphatases Mu, Kappa and PCP-2 (EC 3.1.3.48). These PTPases have an extracellular region which consists of a MAM domain followed by an Ig-like domain and four fibronectin-type III domains.
  • Vertebrate enteropeptidase (EC 3.4.21.9), a type II membrane protein of the intestinal brush border, which activates trypsinogen. It consists at least of a catalytic light chain and a multidomain heavy chain which has 2 LDL receptor class A domains (see <PDOC00929>), a MAM domain, a SRCR domain (see <PDOC00348>) and a CUB domain (see <PDOC00604>).
  • Apical endosomal glycoprotein from rat, a protein probably involved in the sorting and selective transport of receptors and ligands across polarized epithelia. This protein contains 6 MAM domains.
  • Xenopus laevis thyroid hormone induced protein B. This protein contains 4 MAM domains.
  • Pig zonadhesin, a protein that binds in a species-specific manner to the zona pellucida of the egg.

The MAM domain is likely to have an adhesive function. It contains four conserved cysteines which probably form two disulfide bridges. The pattern for MAM contains the third conserved cysteine which is located in the central part of the domain.

Expert(s) to contact by email:

Bork P.

Last update:

December 2004 / Pattern and text revised.

Technical section

PROSITE methods (with tools and information) covered by this documentation:

MAM_2, PS50060; MAM domain profile  (MATRIX)
Sequences known to belong to this class detected by the profile: ALL
Other sequence(s) detected in Swiss-Prot: NONE.
• Domain architecture view of Swiss-Prot proteins matching PS50060
PS50060
• Retrieve an alignment of Swiss-Prot true positive hits:
  Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS50060
• Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS50060
• Scan Swiss-Prot/TrEMBL entries against PS50060
• view ligand binding statistics
Matching PDB structures: 2C9A 2V5Y 4GWM 4GWN [ALL]
MAM_1, PS00740; MAM domain signature  (PATTERN)
Consensus pattern: [GE]-x-[LIVMFY](2)-x(3)-[STA]-x(10,11)-[LV]-x(4)-[LIVMF]-x(6,7)-C-[LIVM]-x-F-x-[LIVMFY]-x(3)-[GSC]
C may be involved in a disulfide bond
Sequences known to belong to this class detected by the pattern: ALL, except for apical endosomal glycoprotein from rat
Other sequence(s) detected in Swiss-Prot: NONE.
• Retrieve an alignment of Swiss-Prot true positive hits:
  Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS00740
• Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS00740
• Scan Swiss-Prot/TrEMBL entries against PS00740
• view ligand binding statistics
Matching PDB structures: 2V5Y 4GWM 4GWN [ALL]

Reference

1 Authors Beckmann G., Bork P.
Title An adhesive domain detected in functionally diverse receptors.
Source Trends Biochem. Sci. 18:40-41(1993).
PubMed ID 8387703

Copyright

PROSITE is copyright. It is produced by the SIB Swiss Institute Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified. Usage by and for commercial entities requires a license agreement. For information about the licensing scheme send an email to
Prosite License or see: prosite_license.html.

Miscellaneous

View entry in original PROSITE document format
View entry in raw text format (no links)