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	PROSITE documentation PDOC00614

A conserved domain of 46 amino acids has been shown [1] to exist in a number of higher eukaryote aminoacyl-transfer RNA synthetases. This domain is present one to six times in the following enzymes:

• Mammalian multifunctional aminoacyl-tRNA synthetase. The domain is present three times in a region that separates the N-terminal glutamyl-tRNA synthetase domain from the C-terminal prolyl-tRNA synthetase domain.
• Drosophila multifunctional aminoacyl-tRNA synthetase. The domain is present six times in the intercatalytic region.
• Mammalian tryptophanyl-tRNA synthetase. The domain is found at the N- terminal extremity.
• Mammalian, insect, nematode and plant glycyl-tRNA synthetase. The domain is found at the N-terminal extremity [2].
• Mammalian histidyl-tRNA synthetase. The domain is found at the N-terminal extremity.

The structure of a human WHEP-TRS domain has been solved by NMR (see <PDB:1FYJ>) [3]. It consists of two helices arranged in a helix-turn-helix. This domain may play a role in the association of tRNA-synthetases into multienzyme complexes [4].

We developed a signature pattern based on the first 29 positions of the WHEP-domain. We also developed a profile that covers the whole WHEP-TRS domain.

Last update:

April 2006 / Pattern revised.

PROSITE methods (with tools and information) covered by this documentation:

WHEP_TRS_2, PS51185; WHEP-TRS domain profile  (MATRIX)

• Sequences in UniProtKB/Swiss-Prot known to belong to this class: 29
  • detected by PS51185: 28 (true positives)
  • undetected by PS51185: 1 (0 false negative and 1 'partial')
• Other sequence(s) in UniProtKB/Swiss-Prot detected by PS51185:
  NONE.
• Domain architecture view of Swiss-Prot proteins matching PS51185
  
• Retrieve an alignment of UniProtKB/Swiss-Prot true positive hits:
  Clustal format, color, condensed view  /  Clustal format, color  /  Clustal format, plain text  /  Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic distribution of all UniProtKB (Swiss-Prot + TrEMBL) entries matching PS51185
• Retrieve a list of all UniProtKB (Swiss-Prot + TrEMBL) entries matching PS51185
• Scan UniProtKB (Swiss-Prot and/or TrEMBL) entries against PS51185
• View ligand binding statistics of PS51185
• Matching PDB structures: 1D2D 1FYJ 1R1B 1R6T ... [ALL]

WHEP_TRS_1, PS00762; WHEP-TRS domain signature  (PATTERN)

• Consensus pattern:
  [QYR]-[GH]-[DNEAR]-x-[LIV]-[KR]-x(2)-K-x(2)-[KRNG]-[AS]-x(4)-[LIV]-[DENKA]-x(2)-[IV]-x(2)-L-x(3)-K
• Sequences in UniProtKB/Swiss-Prot known to belong to this class: 29
  • detected by PS00762: 25 (true positives)
  • undetected by PS00762: 4 (2 false negatives and 2 'partials')
• Other sequence(s) in UniProtKB/Swiss-Prot detected by PS00762:
  NONE.
• Retrieve an alignment of UniProtKB/Swiss-Prot true positive hits:
  Clustal format, color, condensed view  /  Clustal format, color  /  Clustal format, plain text  /  Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic distribution of all UniProtKB (Swiss-Prot + TrEMBL) entries matching PS00762
• Retrieve a list of all UniProtKB (Swiss-Prot + TrEMBL) entries matching PS00762
• Scan UniProtKB (Swiss-Prot and/or TrEMBL) entries against PS00762
• View ligand binding statistics of PS00762
• Matching PDB structures: 1FYJ 1R6T 1X59 2DJV ... [ALL]

1	Authors	Cerini C., Kerjan P., Astier M., Gratecos D., Mirande M., Semeriva M.
	Title	A component of the multisynthetase complex is a multifunctional aminoacyl-tRNA synthetase.
	Source	EMBO J. 10:4267-4277(1991).
	PubMed ID	1756734

2	Authors	Nada S., Chang P.K., Dignam J.D.
	Title	Primary structure of the gene for glycyl-tRNA synthetase from Bombyx mori.
	Source	J. Biol. Chem. 268:7660-7667(1993).
	PubMed ID	8463296

3	Authors	Jeong E.J., Hwang G.S., Kim K.H., Kim M.J., Kim S., Kim K.S.
	Title	Structural analysis of multifunctional peptide motifs in human bifunctional tRNA synthetase: identification of RNA-binding residues and functional implications for tandem repeats.
	Source	Biochemistry 39:15775-15782(2000).
	PubMed ID	11123902
	DOI	10.1021/bi001393h

4	Authors	Rho S.B., Lee J.S., Jeong E.J., Kim K.S., Kim Y.G., Kim S.
	Title	A multifunctional repeated motif is present in human bifunctional tRNA synthetase.
	Source	J. Biol. Chem. 273:11267-11273(1998).
	PubMed ID	9556618

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