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| PROSITE documentation PDOC00628 |
Chorismate synthase signatures
Description:
Chorismate synthase (EC 4.2.3.5) catalyzes the last of the seven steps in the
shikimate pathway which is used in prokaryotes, fungi and plants for the
biosynthesis of aromatic amino acids. It catalyzes the 1,4-trans elimination
of the phosphate group from 5-enolpyruvylshikimate-3-phosphate (EPSP) to form
chorismate which can then be used in phenylalanine, tyrosine or tryptophan
biosynthesis. Chorismate synthase requires the presence of a reduced flavin
mononucleotide (FMNH2 or FADH2) for its activity.
Chorismate synthase from various sources shows [1,2] a high degree of sequence
conservation. It is a protein of about 360 to 400 amino-acid residues. We
developed three signature patterns from conserved regions rich in basic
residues (mostly arginines). The first is in the N-terminal section, the
second is central and the third is C-terminal.
Last update:
April 2006 / Pattern revised.
Technical section:
PROSITE methods (with tools and information) covered by this documentation:
| CHORISMATE_SYNTHASE_1, PS00787; Chorismate synthase signature 1 (PATTERN) |
| Consensus pattern: |
G-[DES]-S-H-[GC]-x(2)-[LIVM]-[GTIVLAMS]-x-[LIVTM]-[LIVM]-[DEST]-[GH]-x-[PV]
|
| Sequences known to belong to this class detected by the pattern: |
ALL |
| Other sequence(s) detected in Swiss-Prot: |
NONE. |
|
|
|
| Matching PDB structures:
1Q1L 1QXO 1R52 1R53 ... [ALL] |
| CHORISMATE_SYNTHASE_2, PS00788; Chorismate synthase signature 2 (PATTERN) |
| Consensus pattern: |
[GE]-x(2)-S-[AG]-R-x-[ST]-x(3)-[VT]-x(2)-[GA]-[STAVY]-[LIVMF]
|
| Sequences known to belong to this class detected by the pattern: |
ALL |
| Other sequence(s) detected in Swiss-Prot: |
NONE. |
|
|
|
| Matching PDB structures:
1Q1L 1QXO 1UM0 1UMF ... [ALL] |
| CHORISMATE_SYNTHASE_3, PS00789; Chorismate synthase signature 3 (PATTERN) |
| Consensus pattern: |
R-[SHF]-D-[PSV]-[CSAVT]-x(4)-[SGAIVM]-x-[IVGSTAPM]-[LIVM]-x-E-[STAHNCG]-[LIVMA]
|
| Sequences known to belong to this class detected by the pattern: |
ALL |
| Other sequence(s) detected in Swiss-Prot: |
NONE. |
|
|
|
| Matching PDB structures:
1R52 1R53 1UM0 1UMF ... [ALL] |
References:
| 1 |
Authors | Schaller A., Schmid J., Leibinger U., Amrhein N. |
| Title | Molecular cloning and analysis of a cDNA coding for chorismate synthase from the higher plant Corydalis sempervirens Pers. |
| Source | J. Biol. Chem. 266:21434-21438(1991). |
| PubMed ID | 1718979 |
| 2 |
Authors | Jones D.G.L., Reusser U., Braus G.H. |
| Title | Molecular cloning, characterization and analysis of the regulation of the ARO2 gene, encoding chorismate synthase, of Saccharomyces cerevisiae. |
| Source | Mol. Microbiol. 5:2143-2152(1991). |
| PubMed ID | 1837329 |
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