|PROSITE documentation PDOC00641
Eukaryotic initiation factor 4E signature
Eukaryotic translation initiation factor 4E (eIF-4E)  is a protein that
binds to the cap structure of eukaryotic cellular mRNAs. eIF-4E recognizes and
binds the 7-methylguanosine-containing (m7Gppp) cap during an early step in
the initiation of protein synthesis and facilitates ribosome binding to a mRNA
by inducing the unwinding of its secondary structures.
eIF-4E is a conserved protein of about 25 Kd. Site directed mutagenesis
experiments have shown  that a tryptophan in the central part of the
sequence of human eIF-4E seems to be implicated in cap-binding. The signature
pattern for eIF-4E includes this tryptophan.
December 2004 / Pattern and text revised.
PROSITE method (with tools and information) covered by this documentation:
|IF4E, PS00813; Eukaryotic initiation factor 4E signature (PATTERN)
The first W seems to be involved in cap-binding
|Sequences known to belong to this class detected by the pattern:
|Other sequence(s) detected in Swiss-Prot:
|Matching PDB structures:
1AP8 1EJ1 1EJ4 1EJH ... [ALL]
||Cap recap: the involvement of eIF-4F in regulating gene expression.
||Ueda H., Iyo H., Doi M., Inoue M., Ishida T., Morioka H., Tanaka T., Nishikawa S., Uesugi S.
||Combination of Trp and Glu residues for recognition of mRNA cap structure. Analysis of m7G base recognition site of human cap binding protein (IF-4E) by site-directed mutagenesis.
||FEBS Lett. 280:207-210(1991).
PROSITE is copyright. It is produced by the SIB Swiss Institute
Bioinformatics. There are no restrictions on its use by non-profit
institutions as long as its content is in no way modified. Usage by and
for commercial entities requires a license agreement. For information
about the licensing scheme send an email to
View entry in original PROSITE document format
View entry in raw text format (no links)