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Eukaryotic translation initiation factor 4E (eIF-4E) [1] is a protein that binds to the cap structure of eukaryotic cellular mRNAs. eIF-4E recognizes and binds the 7-methylguanosine-containing (m7Gppp) cap during an early step in the initiation of protein synthesis and facilitates ribosome binding to a mRNA by inducing the unwinding of its secondary structures.

eIF-4E is a conserved protein of about 25 Kd. Site directed mutagenesis experiments have shown [2] that a tryptophan in the central part of the sequence of human eIF-4E seems to be implicated in cap-binding. The signature pattern for eIF-4E includes this tryptophan.

December 2004 / Pattern and text revised.

PROSITE method (with tools and information) covered by this documentation:

IF4E, PS00813; Eukaryotic initiation factor 4E signature  (PATTERN)

Consensus pattern: [DE]-[IFYL]-x(2)-F-[KRL]-x(2)-[LIVM]-x-P-x-W-E-[DVA]-x(5)-G-G-[KR]-W The first W seems to be involved in cap-binding Sequences known to belong to this class detected by the pattern: ALL Other sequence(s) detected in Swiss-Prot: NONE

• Retrieve an alignment of Swiss-Prot true positive hits:   Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format • Retrieve the sequence logo from the alignment • Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS00813 • Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS00813 • Scan Swiss-Prot/TrEMBL entries against PS00813 • view ligand binding statistics

Matching PDB structures: 1AP8 1EJ1 1EJ4 1EJH ... [ALL]

1	Authors	Thach R.E.
	Title	Cap recap: the involvement of eIF-4F in regulating gene expression.
	Source	Cell 68:177-180(1992).
	PubMed ID	1733496

2	Authors	Ueda H., Iyo H., Doi M., Inoue M., Ishida T., Morioka H., Tanaka T., Nishikawa S., Uesugi S.
	Title	Combination of Trp and Glu residues for recognition of mRNA cap structure. Analysis of m7G base recognition site of human cap binding protein (IF-4E) by site-directed mutagenesis.
	Source	FEBS Lett. 280:207-210(1991).
	PubMed ID	1672854

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