{PDOC00646} {PS00820; GLUCOAMYLASE} {BEGIN} ********************************************* * Glucoamylase active site region signature * ********************************************* Glucoamylase (GA) (EC 3.2.1.3) is an enzyme that catalyzes the release of D- glucose from the non-reducing ends of starch and other oligo- or polysaccharides. Extensive studies of fungal GA have shown [1,E1] that three closely clustered acidic residues play a role in the catalytic mechanism of GA. The region that includes these residues is also conserved in a recently sequenced bacterial GA [2]. We used this region as a signature pattern. -Consensus pattern: [STN]-[GP]-x(1,2)-[DE]-x-W-E-E-x(2)-[GS] [All D/E are active site residues] -Sequences known to belong to this class detected by the pattern: ALL. -Other sequence(s) detected in Swiss-Prot: 1. -Note: These proteins belong to family 15 in the classification of glycosyl hydrolases [3,E1]. -Note: A GA from Schwanniomyces occidentalis is not a member of this family but belongs to family 31 (see ). -Last update: July 1998 / Text revised. [ 1] Sierks M.R., Ford C., Reilly P.J., Svensson B. "Catalytic mechanism of fungal glucoamylase as defined by mutagenesis of Asp176, Glu179 and Glu180 in the enzyme from Aspergillus awamori." Protein Eng. 3:193-198(1990). PubMed=1970434 [ 2] Ohnishi H., Kitamura H., Minowa T., Sakai H., Ohta T. "Molecular cloning of a glucoamylase gene from a thermophilic Clostridium and kinetics of the cloned enzyme." Eur. J. Biochem. 207:413-418(1992). PubMed=1633799 [ 3] Henrissat B. "A classification of glycosyl hydrolases based on amino acid sequence similarities." Biochem. J. 280:309-316(1991). PubMed=1747104 [E1] http://www.cazy.org/GH15.html -------------------------------------------------------------------------------- PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see https://prosite.expasy.org/prosite_license.html -------------------------------------------------------------------------------- {END}