{PDOC00701}
{PS00902; GLUTAMATE_5_KINASE}
{BEGIN}
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* Glutamate 5-kinase signature *
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Glutamate 5-kinase  (EC 2.7.2.11) (gamma-glutamyl kinase) (GK)  is  the enzyme
that catalyzes  the  first step in the biosynthesis of proline from glutamate,
the ATP-dependent phosphorylation of L-glutamate into L-glutamate 5-phosphate.

In eubacteria  (gene  proB)  and yeast [1] (gene PRO1), GK is a monofunctional
protein, while  in plants  and mammals, it is a bifunctional enzyme (P5CS) [2]
that consists  of  two domains: a N-terminal GK domain and a C-terminal gamma-
glutamyl phosphate reductase domain (EC 1.2.1.41) (see <PDOC00940>).

As a  signature  pattern,  we selected a highly conserved glycine-and alanine-
rich region located in the central section of these enzymes.

Yeast hypothetical protein YHR033w is highly similar to GK.

-Consensus pattern: [GSTNAD]-x(2)-[GAS]-x-G-[GC]-[IM]-x-[STAG]-K-[LIVMCT]-x-
                    [SAI]-[TCAGFS]-x(2)-[GALVCMI]
-Sequences known to belong to this class detected by the pattern: ALL.
-Other sequence(s) detected in Swiss-Prot: NONE.
-Last update: December 2004 / Pattern and text revised.

[ 1] Li W., Brandriss M.C.
     "Proline biosynthesis in Saccharomyces cerevisiae: molecular analysis
     of the PRO1 gene, which encodes gamma-glutamyl kinase."
     J. Bacteriol. 174:4148-4156(1992).
     PubMed=1350780
[ 2] Hu C.-A.A., Delauney A.J., Verma D.P.S.
     "A bifunctional enzyme (delta 1-pyrroline-5-carboxylate synthetase)
     catalyzes the first two steps in proline biosynthesis in plants."
     Proc. Natl. Acad. Sci. U.S.A. 89:9354-9358(1992).
     PubMed=1384052

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