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| PROSITE documentation PDOC00702 |
Cytidine and deoxycytidylate deaminases zinc-binding region signature
Description:
Cytidine deaminase (EC 3.5.4.5) (cytidine aminohydrolase) catalyzes the
hydrolysis of cytidine into uridine and ammonia while deoxycytidylate
deaminase (EC 3.5.4.12) (dCMP deaminase) hydrolyzes dCMP into dUMP. Both
enzymes are known to bind zinc and to require it for their catalytic activity
[1,2]. These two enzymes do not share any sequence similarity with the
exception of a region that contains three conserved histidine and cysteine
residues which are thought to be involved in the binding of the catalytic zinc
ion.
Such a region is also found in other proteins [3,4]:
- Yeast cytosine deaminase (EC 3.5.4.1) (gene FCY1) which transforms cytosine
into uracil.
- Mammalian apolipoprotein B mRNA editing protein, responsible for the
postranscriptional editing of a CAA codon into a UAA (stop) codon in the
APOB mRNA.
- Riboflavin biosynthesis protein ribG, which converts 2,5-diamino-6-
(ribosylamino)-4(3H)-pyrimidinone 5'-phosphate into 5-amino-6-
(ribosylamino)-2,4(1H,3H)-pyrimidinedione 5'-phosphate.
- Bacillus cereus blasticidin-S deaminase (EC 3.5.4.23), which catalyzes the
deamination of the cytosine moiety of the antibiotics blasticidin S,
cytomycin and acetylblasticidin S.
- Bacillus subtilis protein comEB. This protein is required for the binding
and uptake of transforming DNA.
- Bacillus subtilis hypothetical protein yaaJ.
- Escherichia coli hypothetical protein yfhC.
- Yeast hypothetical protein YJL035c.
We have derived a signature pattern for this zinc-binding region.
Last update:
May 2004 / Text revised.
Technical section:
PROSITE method (with tools and information) covered by this documentation:
| CYT_DCMP_DEAMINASES, PS00903; Cytidine and deoxycytidylate deaminases zinc-binding region signature (PATTERN) |
| Consensus pattern: |
[CH]-[AGV]-E-x(2)-[LIVMFGAT]-[LIVM]-x(17,33)-P-C-x(2,8)-C-x(3)-[LIVM]
The C's and H are zinc ligands |
| Sequences known to belong to this class detected by the pattern: |
ALL |
| Other sequence(s) detected in Swiss-Prot: |
1 |
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| Matching PDB structures:
1AF2 1ALN 1CTT 1CTU ... [ALL] |
References:
| 1 |
Authors | Yang C., Carlow D., Wolfenden R., Short S.A. |
| Title | Cloning and nucleotide sequence of the Escherichia coli cytidine deaminase (ccd) gene. |
| Source | Biochemistry 31:4168-4174(1992). |
| PubMed ID | 1567863 |
| 2 |
Authors | Moore J.T., Silversmith R.E., Maley G.F., Maley F. |
| Title | T4-phage deoxycytidylate deaminase is a metalloprotein containing two zinc atoms per subunit. |
| Source | J. Biol. Chem. 268:2288-2291(1993). |
| PubMed ID | 8428902 |
| 3 |
Authors | Reizer J., Buskirk S., Bairoch A., Reizer A., Saier M.H. Jr. |
| Title | A novel zinc-binding motif found in two ubiquitous deaminase families. |
| Source | Protein Sci. 3:853-856(1994). |
| PubMed ID | 8061614 |
| 4 |
Authors | Bhattacharya S., Navaratnam N., Morrison J.R., Scott J., Taylor W.R. |
| Title | Cytosine nucleoside/nucleotide deaminases and apolipoprotein B mRNA editing. |
| Source | Trends Biochem. Sci. 19:105-106(1994). |
| PubMed ID | 8203015 |
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