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PROSITE documentation PDOC00708

Dihydroorotate dehydrogenase signatures

Description:

Dihydroorotate dehydrogenase (EC 1.3.3.1) (DHOdehase) catalyzes the fourth step in the de novo biosynthesis of pyrimidine, the conversion of dihydroorotate into orotate. DHOdehase is a ubiquitous FAD flavoprotein.

In bacteria (gene pyrD), DHOdease is located on the inner side of the cytosolic membrane. In some yeasts, such as in Saccharomyces cerevisiae (gene URA1), it is a cytosolic protein while in other eukaryotes it is found in the mitochondria [1].

The sequence of DHOdease is rather well conserved and we have developed two signature patterns specific to this enzyme. The first corresponds to a region in the N-terminal section of the enzyme while the second is located in the C-terminal section and seems to be part of the FAD-binding domain.

Last update:

April 2006 / Pattern revised.

Technical section:

PROSITE methods (with tools and information) covered by this documentation:

DHODEHASE_1, PS00911; Dihydroorotate dehydrogenase signature 1  (PATTERN)
Consensus pattern: [GSA]-x(4)-[GK]-[GSTA]-[LIVFSTA]-[GST]-x(3)-[NQRK]-x-G-[NHY]-x(2)-P-[RTV]
Sequences known to belong to this class detected by the pattern: ALL
Other sequence(s) detected in Swiss-Prot: NONE.
• Retrieve an alignment of Swiss-Prot true positive hits:
  Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS00911
• Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS00911
• Scan Swiss-Prot/TrEMBL entries against PS00911
• view ligand binding statistics
Matching PDB structures: 1D3G 1D3H 1DOR 1EP1 ... [ALL]
DHODEHASE_2, PS00912; Dihydroorotate dehydrogenase signature 2  (PATTERN)
Consensus pattern: [LIVM](2)-[GSA]-x-G(2)-[IV]-x-[STGDN]-x(3)-[ACV]-x(2)-{A}-{R}-x-{L}-G-A
Sequences known to belong to this class detected by the pattern: ALL
Other sequence(s) detected in Swiss-Prot: 10.
• Retrieve an alignment of Swiss-Prot true positive hits:
  Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS00912
• Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS00912
• Scan Swiss-Prot/TrEMBL entries against PS00912
• view ligand binding statistics
Matching PDB structures: 1D3G 1D3H 1DOR 1EP1 ... [ALL]

Reference:

1 AuthorsNagy M., Lacroute F., Thomas D.
TitleDivergent evolution of pyrimidine biosynthesis between anaerobic and aerobic yeasts.
SourceProc. Natl. Acad. Sci. U.S.A. 89:8966-8970(1992).
PubMed ID1409592

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