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PROSITE documentation PDOC00714

Aspartate and glutamate racemases signatures

Description:

Aspartate racemase (EC 5.1.1.13) and glutamate racemase (EC 5.1.1.3) are two evolutionary related bacterial enzymes that do not seem to require a cofactor for their activity [1]. Glutamate racemase, which interconverts L-glutamate into D-glutamate, is required for the biosynthesis of peptidoglycan and some peptide-based antibiotics such as gramicidin S.

In addition to characterized aspartate and glutamate racemases, this family also includes a hypothetical protein from Erwinia carotovora and one from Escherichia coli (ygeA).

Two conserved cysteines are present in the sequence of these enzymes. They are expected to play a role in catalytic activity by acting as bases in proton abstraction from the substrate. We developed signature patterns for both cysteines.

Last update:

May 2004 / Text revised.

Technical section:

PROSITE methods (with tools and information) covered by this documentation:

ASP_GLU_RACEMASE_1, PS00923; Aspartate and glutamate racemases signature 1  (PATTERN)
Consensus pattern: [IVA]-[LIVM]-x-C-x(0,1)-N-[ST]-[MSA]-[STH]-[LIVFYSTANK]
The C is an active site residue
Sequences known to belong to this class detected by the pattern: ALL
Other sequence(s) detected in Swiss-Prot: 5.
• Retrieve an alignment of Swiss-Prot true positive hits:
  Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS00923
• Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS00923
• Scan Swiss-Prot/TrEMBL entries against PS00923
• view ligand binding statistics
Matching PDB structures: 1B73 1B74 1ZUW 2JFN ... [ALL]
ASP_GLU_RACEMASE_2, PS00924; Aspartate and glutamate racemases signature 2  (PATTERN)
Consensus pattern: [LIVM](2)-x-[AG]-C-T-[DEH]-[LIVMFY]-[PNGRS]-x-[LIVM]
The C is an active site residue
Sequences known to belong to this class detected by the pattern: ALL
Other sequence(s) detected in Swiss-Prot: NONE.
• Retrieve an alignment of Swiss-Prot true positive hits:
  Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS00924
• Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS00924
• Scan Swiss-Prot/TrEMBL entries against PS00924
• view ligand binding statistics
Matching PDB structures: 1B73 1B74 1ZUW 2JFN ... [ALL]

Reference:

1 AuthorsGallo K.A., Knowles J.R.
TitlePurification, cloning, and cofactor independence of glutamate racemase from Lactobacillus.
SourceBiochemistry 32:3981-3990(1993).
PubMed ID8385993

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