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| PROSITE documentation PDOC00746 |
Phosphomannose isomerase type I signatures
Description:
Phosphomannose isomerase (EC 5.3.1.8) (PMI) [1,2] is the enzyme that catalyzes
the interconversion of mannose-6-phosphate and fructose-6-phosphate. In
eukaryotes, it is involved in the synthesis of GDP-mannose which is a
constituent of N- and O-linked glycans as well as GPI anchors. In prokaryotes,
it is involved in a variety of pathways including capsular polysaccharide
biosynthesis and D-mannose metabolism.
Three classes of PMI have been defined on the basis of sequence similarities
[1]. The first class comprises all known eukaryotic PMI as well as the enzyme
encoded by the manA gene in enterobacteria such as Escherichia coli. Class I
PMI's are proteins of about 42 to 50 Kd which bind a zinc ion essential for
their activity.
As signature patterns for class I PMI, we selected two conserved regions. The
first one is located in the N-terminal section of these proteins, the second
in the C-terminal half. Both patterns contain a residue involved [3] in the
binding of the zinc ion.
Expert(s) to contact by email:
Proudfoot A.E.I.
Last update:
November 1997 / Text revised.
Technical section:
PROSITE methods (with tools and information) covered by this documentation:
| PMI_I_1, PS00965; Phosphomannose isomerase type I signature 1 (PATTERN) |
| Consensus pattern: |
Y-x-D-x-N-H-K-P-E
E is a zinc ligand |
| Sequences known to belong to this class detected by the pattern: |
ALL |
| Other sequence(s) detected in Swiss-Prot: |
NONE. |
|
|
|
| Matching PDB structures:
1PMI 2WFP 3H1M 3H1W ... [ALL] |
| PMI_I_2, PS00966; Phosphomannose isomerase type I signature 2 (PATTERN) |
| Consensus pattern: |
H-A-Y-[LIVM]-x-G-x(2)-[LIVM]-E-x-M-A-x-S-D-N-x-[LIVM]-R-A-G-x-T-P-K
H is a zinc ligand |
| Sequences known to belong to this class detected by the pattern: |
ALL |
| Other sequence(s) detected in Swiss-Prot: |
NONE. |
|
|
|
| Matching PDB structures:
1PMI 2WFP 3H1M 3H1W ... [ALL] |
References:
| 1 |
Authors | Proudfoot A.E.I., Turcatti G., Wells T.N., Payton M.A., Smith D.J. |
| Title | Purification, cDNA cloning and heterologous expression of human phosphomannose isomerase. |
| Source | Eur. J. Biochem. 219:415-423(1994). |
| PubMed ID | 8307007 |
| 2 |
Authors | Coulin F., Magnenat E., Proudfoot A.E.I., Payton M.A., Scully P., Wells T.N.C. |
| Title | Identification of Cys-150 in the active site of phosphomannose isomerase from Candida albicans. |
| Source | Biochemistry 32:14139-14144(1993). |
| PubMed ID | 8260497 |
| 3 |
Authors | Cleasby A., Wonacott A., Skarzynski T., Hubbard R.E., Davies G.J., Proudfoot A.E.I., Bernard A.R., Payton M.A., Wells T.N.C. |
| Title | The x-ray crystal structure of phosphomannose isomerase from Candida albicans at 1.7 angstrom resolution. |
| Source | Nat. Struct. Biol. 3:470-479(1996). |
| PubMed ID | 8612079 |
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