PROSITE documentation PDOC00750

Ubiquitin carboxyl-terminal hydrolases family 2 signatures/profile

Description

Ubiquitin carboxyl-terminal hydrolases (EC 3.1.2.15) (UCH) (deubiquitinating enzymes) [1,2] are thiol proteases that recognize and hydrolyze the peptide bond at the C-terminal glycine of ubiquitin. These enzymes are involved in the processing of poly-ubiquitin precursors as well as that of ubiquinated proteins.

There are two distinct families of UCH. The second class consist of large proteins (800 to 2000 residues) and is currently represented by:

  • Yeast UBP1, UBP2, UBP3, UBP4 (or DOA4/SSV7), UBP5, UBP7, UBP9, UBP10, UBP11, UBP12, UBP13, UBP14, UBP15 and UBP16.
  • Human tre-2.
  • Human isopeptidase T.
  • Human isopeptidase T-3.
  • Mammalian Ode-1.
  • Mammalian Unp.
  • Mouse Dub-1.
  • Drosophila fat facets protein (gene faf).
  • Mammalian faf homolog.
  • Drosophila D-Ubp-64E.
  • Caenorhabditis elegans hypothetical protein R10E11.3.
  • Caenorhabditis elegans hypothetical protein K02C4.3.

These proteins only share two regions of similarity. The first region contains a conserved cysteine which is probably implicated in the catalytic mechanism. The second region contains two conserved histidines residues, one of which is also probably implicated in the catalytic mechanism. We have developed signature pattern for both conserved regions. We also developed a profile including the two regions covered by the patterns.

Note:

These proteins belong to family C19 in the classification of peptidases [3,E1].

Last update:

April 2006 / Pattern revised.

Technical section

PROSITE methods (with tools and information) covered by this documentation:

UCH_2_3, PS50235; Ubiquitin carboxyl-terminal hydrolases family 2 profile  (MATRIX)
Sequences known to belong to this class detected by the profile: ALL
Other sequence(s) detected in Swiss-Prot: NONE.
• Domain architecture view of Swiss-Prot proteins matching PS50235
PS50235
• Retrieve an alignment of Swiss-Prot true positive hits:
  Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS50235
• Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS50235
• Scan Swiss-Prot/TrEMBL entries against PS50235
• view ligand binding statistics
Matching PDB structures: 1NB8 1NBF 1VJV 2AYN ... [ALL]
UCH_2_1, PS00972; Ubiquitin carboxyl-terminal hydrolases family 2 signature 1  (PATTERN)
Consensus pattern: G-[LIVMFY]-x(1,3)-[AGCY]-[NASMQG]-x-C-[FYWC]-[LIVMFCA]-[NSTAD]-[SACV]-x-[LIVMSF]-[QF]
C may be the active site residue
Sequences known to belong to this class detected by the pattern: ALL, except for two sequences
Other sequence(s) detected in Swiss-Prot: NONE.
• Retrieve an alignment of Swiss-Prot true positive hits:
  Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS00972
• Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS00972
• Scan Swiss-Prot/TrEMBL entries against PS00972
• view ligand binding statistics
Matching PDB structures: 1NBF 1VJV 2AYN 2AYO ... [ALL]
UCH_2_2, PS00973; Ubiquitin carboxyl-terminal hydrolases family 2 signature 2  (PATTERN)
Consensus pattern: [YMF]-x-[LK]-x-[SAGNC]-[LIVMFT]-x(2)-H-x-G-x(4,6)-G-H-[YF]
The 2 H's may be active site residues
Sequences known to belong to this class detected by the pattern: ALL
Other sequence(s) detected in Swiss-Prot: NONE.
• Retrieve an alignment of Swiss-Prot true positive hits:
  Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS00973
• Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS00973
• Scan Swiss-Prot/TrEMBL entries against PS00973
• view ligand binding statistics
Matching PDB structures: 1NB8 1NBF 1VJV 2AYN ... [ALL]

References

1 Authors Jentsch S., Seufert W., Hauser H.-P.
Title Genetic analysis of the ubiquitin system.
Source Biochim. Biophys. Acta 1089:127-139(1991).
PubMed ID 1647207
2 Authors D'andrea A., Pellman D.
Source Crit. Rev. Biochem. Mol. Biol. 33:337-352(1998).
3 Authors Rawlings N.D., Barrett A.J.
Title Families of cysteine peptidases.
Source Methods Enzymol. 244:461-486(1994).
PubMed ID 7845226
E1
Source http://www.uniprot.org/docs/peptidas

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