{PDOC00753} {PS00977; FAD_G3PDH_1} {PS00978; FAD_G3PDH_2} {BEGIN} *************************************************************** * FAD-dependent glycerol-3-phosphate dehydrogenase signatures * *************************************************************** FAD-dependent glycerol-3-phosphate dehydrogenase (EC 1.1.99.5) (GPD) catalyzes the conversion of glycerol-3-phosphate into dihydroxyacetone phosphate. In bacteria [1] it is associated with the utilization of glycerol coupled to respiration. In Escherichia coli, two isozymes are known: one expressed under anaerobic conditions (gene glpA) and one in aerobic conditions (gene glpD). In eukaryotes, a mitochondrial form of GPD participates in the glycerol phosphate shuttle in conjunction with an NAD-dependent cytoplasmic GPD (EC 1.1.1.8) [2, 3]. These enzymes are proteins of about 60 to 70 Kd which contain a probable FAD-binding domain in their N-terminal extremity. The mammalian enzyme differs from the bacterial or yeast proteins by having an EF-hand calcium-binding region (See ) in its C-terminal extremity. We developed two signature patterns. One based on the first half of the FAD- binding domain and one which corresponds to a conserved region in the central part of these enzymes. -Consensus pattern: [IV]-G-G-G-x(2)-G-[STACV]-G-x-[AT]-x-[DQ]-x(3)-[RAS]-G -Sequences known to belong to this class detected by the pattern: ALL. -Other sequence(s) detected in Swiss-Prot: NONE. -Consensus pattern: G-G-K-x(2)-[GSTE]-Y-R-x(2)-A -Sequences known to belong to this class detected by the pattern: ALL. -Other sequence(s) detected in Swiss-Prot: NONE. -Expert(s) to contact by email: Brown L.J.; ljbrown2@facstaff.wisc.edu -Last update: December 2004 / Pattern and text revised. [ 1] Austin D., Larson T.J. "Nucleotide sequence of the glpD gene encoding aerobic sn-glycerol 3-phosphate dehydrogenase of Escherichia coli K-12." J. Bacteriol. 173:101-107(1991). PubMed=1987111 [ 2] Roennow B., Kielland-Brandt M.C. Yeast 9:1121-1130(1993). [ 3] Brown L.J., McDonald M.J., Lehn D.A., Moran S.M. "Sequence of rat mitochondrial glycerol-3-phosphate dehydrogenase cDNA. Evidence for EF-hand calcium-binding domains." J. Biol. Chem. 269:14363-14366(1994). PubMed=8182039 -------------------------------------------------------------------------------- PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see https://prosite.expasy.org/prosite_license.html -------------------------------------------------------------------------------- {END}