{PDOC00761}
{PS00990; CLAT_ADAPTOR_M_1}
{PS00991; CLAT_ADAPTOR_M_2}
{BEGIN}
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* Clathrin adaptor complexes medium chain signatures *
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Clathrin coated  vesicles (CCV) mediate intracellular membrane traffic such as
receptor mediated  endocytosis.  In addition to clathrin, the CCV are composed
of a number of other components including oligomeric complexes which are known
as adaptor  or  clathrin  assembly  proteins  (AP)  complexes [1]. The adaptor
complexes are  believed  to  interact  with  the cytoplasmic tails of membrane
proteins, leading to their selection and concentration. In mammals two type of
adaptor complexes  are  known: AP-1 which is associated with the Golgi complex
and AP-2  which is associated with the plasma membrane. Both AP-1 and AP-2 are
heterotetramers that  consist  of two large chains - the adaptins - (gamma and
beta' in  AP-1; alpha and beta in AP-2); a medium chain (AP47 in AP-1; AP50 in
AP-2) and a small chain (AP19 in AP-1; AP17 in AP-2).

The medium chains  of AP-1 and AP-2 are evolutionary related proteins of about
50 Kd.  Homologs  of  AP47  and  AP50  have  also been found in Caenorhabditis
elegans (genes unc-101 and ap50) [2] and yeast (gene APM1 or YAP54) [3].

Some more divergent, but  clearly evolutionary related proteins have also been
found in yeast: APM2 and YBR288c.

As signature   patterns, we selected two conserved regions, one located in the
N-terminal region, the other from the central section of these proteins.

-Consensus pattern: [IVT]-[GSP]-W-R-x(2,3)-[GAD]-x(2)-[HY]-x(2)-N-x-
                    [LIVMAFY](3)-D-[LIVM]-[LIVMT]-E
-Sequences known to belong to this class detected by the pattern: ALL.
-Other sequence(s) detected in Swiss-Prot: NONE.

-Consensus pattern: [LIV]-x-[FL]-[IQ]-P-P-x-G-x-[LIVMFY]-x-[LV]-x(2)-Y
-Sequences known to belong to this class detected by the pattern: ALL.
-Other sequence(s) detected in Swiss-Prot: NONE.

-Last update: December 2004 / Pattern and text revised.

[ 1] Pearse B.M., Robinson M.S.
     "Clathrin, adaptors, and sorting."
     Annu. Rev. Cell Biol. 6:151-171(1990).
     PubMed=2177341; DOI=10.1146/annurev.cb.06.110190.001055
[ 2] Lee J., Jongeward G.D., Sternberg P.W.
     "unc-101, a gene required for many aspects of Caenorhabditis elegans
     development and behavior, encodes a clathrin-associated protein."
     Genes Dev. 8:60-73(1994).
     PubMed=8288128
[ 3] Nakayama Y., Goebl M., O'Brine G.B., Lemmon S., Pingchang C.E.,
     Kirchhausen T.
     Eur. J. Biochem. 202:569-574(1991).

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