{PDOC00772} {PS01009; CRISP_1} {PS01010; CRISP_2} {BEGIN} ************************** * CRISP family signature * ************************** A variety of extracellular proteins from eukaryotes have been found to be evolutionary related. They have been termed cysteine-rich secretory proteins (CRISP). Some relevant members of this family are: - Rodent sperm-coating glycoprotein (SCP), also known as acidic epididymal glycoprotein (AEG) . This protein is thought to be involved in sperm maturation [1]. It is a protein of about 220 residues and probably contains eight disulfide bonds. - Mammalian testis-specific protein Tpx-1 [2]. Tpx-1 is highly related to SCP's. - Mammalian glioma pathogenesis-related protein (GliPR). - Lizard helothermine, a toxin that blocks ryanodine receptors. - Venom allergen 5 (Ag5) from vespid wasps and venom allergen 3 (Ag3) from fire ants. These proteins are potent allergens and are the main cause of allergic reactions to stings from insects of the hymenoptera family [3]. Ag5/3 are proteins of about 200 residues and contain four disulfide bonds. - Plant pathogenesis proteins of the PR-1 family [4]. These proteins are synthesized during pathogen infection or other stress-related responses. They are proteins of about 130 to 140 residues and probably contain three disulfide bonds. - Proteins Sc7 and Sc14 from the basidomycete fungus Schizophyllum commune. These extracellular proteins are loosely associated with fruit body hyphal walls [5]. Sc7/14 are proteins of about 180 residues and probably contain two disulfide bonds. - Ancylostoma secreted protein from dog hookworm. - Yeast hypothetical proteins YJL078c, YJL079c and YKR013w. The exact function of these proteins is not yet known. As signature patterns, we selected two conserved regions located in their C-terminal half. The second signature contains a cysteine which is known to be involved in a disulfide bond in Ag5. -Consensus pattern: [GDER]-[HR]-[FYWH]-[TVS]-[QA]-[LIVM]-[LIVMA]-W-x(2)-[STN] -Sequences known to belong to this class detected by the pattern: ALL, except 3 sequences. -Other sequence(s) detected in Swiss-Prot: NONE. -Consensus pattern: [LIVMFYH]-[LIVMFY]-x-C-[NQRHS]-Y-x-[PARH]-x-[GL]-N- [LIVMFYWDN] [C is involved in a disulfide bond] -Sequences known to belong to this class detected by the pattern: ALL, except 4 sequences. -Other sequence(s) detected in Swiss-Prot: NONE. -Last update: April 2006 / Pattern revised. [ 1] Mizuki N., Kasahara M. "Mouse submandibular glands express an androgen-regulated transcript encoding an acidic epididymal glycoprotein-like molecule." Mol. Cell. Endocrinol. 89:25-32(1992). PubMed=1301383 [ 2] Kasahara M., Gutknecht J., Brew K., Spurr N., Goodfellow P.N. "Cloning and mapping of a testis-specific gene with sequence similarity to a sperm-coating glycoprotein gene." Genomics 5:527-534(1989). PubMed=2613236 [ 3] Lu G., Villalba M., Coscia M.R., Hoffman D.R., King T.P. "Sequence analysis and antigenic cross-reactivity of a venom allergen, antigen 5, from hornets, wasps, and yellow jackets." J. Immunol. 150:2823-2830(1993). PubMed=8454859 [ 4] Dixon D.C., Cutt J.R., Klessig D.F. "Differential targeting of the tobacco PR-1 pathogenesis-related proteins to the extracellular space and vacuoles of crystal idioblasts." EMBO J. 10:1317-1324(1991). PubMed=2026137 [ 5] Schuren F.H.J., Asgeirsdottir S.A., Kothe E.M., Scheer J.M.J., Wessels J.G. "The Sc7/Sc14 gene family of Schizophyllum commune codes for extracellular proteins specifically expressed during fruit-body formation." J. Gen. Microbiol. 139:2083-2090(1993). PubMed=8245835 -------------------------------------------------------------------------------- PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see https://prosite.expasy.org/prosite_license.html -------------------------------------------------------------------------------- {END}