PROSITE documentation PDOC00783

Coproporphyrinogen III oxidase signature

Description:

Coproporphyrinogen III oxidase (EC 1.3.3.3) (coproporphyrinogenase) [1,2] catalyzes the oxidative decarboxylation of coproporphyrinogen III into protoporphyrinogen IX, a common step in the pathway for the biosynthesis of porphyrins such as heme, chlorophyll or cobalamin.

Coproporphyrinogen III oxidase is an enzyme that requires iron for its activity. A cysteine seems to be important for the catalytic mechanism [3]. Sequences from a variety of eukaryotic and prokaryotic sources show that this enzyme has been evolutionarily conserved. As a signature pattern we selected a highly conserved region in the central part of the sequence; this region contains the only conserved cysteine and is rich in charged amino acids.

Note:

Some bacteria possess an anaerobic form of coproporphyrinogen III oxidase [4] which does not belong to this family.

Last update:

April 2006 / Pattern revised.

Technical section:

PROSITE method (with tools and information) covered by this documentation:

COPROGEN_OXIDASE, PS01021Coproporphyrinogen III oxidase signature  (PATTERN)
Consensus pattern: K-x-[WQA]-[CA]-x(2)-[FYH](2)-x-[LIVM]-x-[HY]-R-x-E-x-R-G-[LIVMT]-G(2)-[LIVM]-F-[FY]-D
Sequences known to belong to this class detected by the pattern: ALL
Other sequence(s) detected in Swiss-Prot: NONE.
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Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS01021
Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS01021
Scan Swiss-Prot/TrEMBL entries against PS01021
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Matching PDB structures: 1TKL 1TLB 1TXN 1VJU ... [ALL]

References:

1 AuthorsXu K., Elliott T.
TitleAn oxygen-dependent coproporphyrinogen oxidase encoded by the hemF gene of Salmonella typhimurium.
SourceJ. Bacteriol. 175:4990-4999(1993).
PubMed ID8349542
2 AuthorsKohno H., Furukawa T., Yoshinaga T., Tokunaga R., Taketani S.
TitleCoproporphyrinogen oxidase. Purification, molecular cloning, and induction of mRNA during erythroid differentiation.
SourceJ. Biol. Chem. 268:21359-21363(1993).
PubMed ID8407975
3 AuthorsCamadro J.M., Chambon H., Jolles J., Labbe P.
TitlePurification and properties of coproporphyrinogen oxidase from the yeast Saccharomyces cerevisiae.
SourceEur. J. Biochem. 156:579-587(1986).
PubMed ID3516695
4 AuthorsXu K., Elliott T.
TitleCloning, DNA sequence, and complementation analysis of the Salmonella typhimurium hemN gene encoding a putative oxygen-independent coproporphyrinogen III oxidase.
SourceJ. Bacteriol. 176:3196-3203(1994).
PubMed ID8195073

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