|PROSITE documentation PDOC00798
Bacterial extracellular solute-binding proteins, family 3 signature
Bacterial high affinity transport systems are involved in active transport of
solutes across the cytoplasmic membrane. The protein components of these
traffic systems include one or two transmembrane protein components, one or
two membrane-associated ATP-binding proteins (ABC transporters; see
<PDOC00185>) and a high affinity periplasmic solute-binding protein. The later
are thought to bind the substrate in the vicinity of the inner membrane, and
to transfer it to a complex of inner membrane proteins for concentration into
In gram-positive bacteria which are surrounded by a single membrane and have
therefore no periplasmic region the equivalent proteins are bound to the
membrane via an N-terminal lipid anchor. These homolog proteins do not play an
integral role in the transport process per se, but probably serve as receptors
to trigger or initiate translocation of the solute throught the membrane by
binding to external sites of the integral membrane proteins of the efflux
In addition at least some solute-binding proteins function in the initiation
of sensory transduction pathways.
On the basis of sequence similarities, the vast majority of these solute-binding proteins can be grouped  into eight families of clusters, which
generally correlate with the nature of the solute bound.
Family 3 groups together specific amino acids and opine-binding periplasmic
proteins and a periplasmic homolog with catalytic activity:
- Histidine-binding protein (gene hisJ) of Escherichia coli and related
bacteria. An homologous lipoprotein exists in Neisseria gonorrhoeae.
- Lysine/arginine/ornithine-binding proteins (LAO) (gene argT) of Escherichia
coli and related bacteria are involved in the same transport system than
hisJ. Both solute-binding proteins interact with a common membrane-bound
receptor hisP of the binding protein dependent transport system HisQMP.
- Glutamine-binding proteins (gene glnH) of Escherichia coli and Bacillus
- Glutamate-binding protein (gene gluB) of Corynebacterium glutamicum.
- Arginine-binding proteins artI and artJ of Escherichia coli.
- Nopaline-binding protein (gene nocT) from Agrobacterium tumefaciens.
- Octopine-binding protein (gene occT) from Agrobacterium tumefaciens.
- Major cell-binding factor (CBF1) (gene: peb1A) from Campylobacter jejuni.
- Bacteroides nodosus protein aabA.
- Cyclohexadienyl/arogenate dehydratase of Pseudomonas aeruginosa, a
periplasmic enzyme which forms an alternative pathway for phenylalanine
- Escherichia coli protein fliY.
- Vibrio harveyi protein patH.
- Escherichia coli hypothetical protein ydhW.
- Bacillus subtilis hypothetical protein yckB.
- Bacillus subtilis hypothetical protein yckK.
The signature pattern is located near the N-terminus of the mature proteins.
April 2006 / Pattern revised.
PROSITE method (with tools and information) covered by this documentation:
|SBP_BACTERIAL_3, PS01039; Bacterial extracellular solute-binding proteins, family 3 signature (PATTERN)
|Sequences in UniProtKB/Swiss-Prot known to belong to this class detected by PS01039:
||38 true positives with 7 false negatives.
|Other sequence(s) in UniProtKB/Swiss-Prot detected by PS01039:
||29 false positives.
|Matching PDB structures:
1GGG 1HPB 1HSL 1LAF ... [ALL]
||Tam R., Saier M.H. Jr.
||Structural, functional, and evolutionary relationships among extracellular solute-binding receptors of bacteria.
||Microbiol. Rev. 57:320-346(1993).
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