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PROSITE documentation PDOC00820

Prokaryotic diacylglycerol kinase signature

Description:

Diacylglycerol kinase (EC 2.7.1.107) (DAGK) is an enzyme that catalyzes the formation of phosphatidic acid from diacylglycerol and ATP, an important step in phospholipid biosynthesis. In bacteria DAGK is very small (13 to 15 Kd) membrane protein which seems to contain three transmembrane domains [1].

As a signature for bacterial DAGK, we selected the best conserved region, a stretch of 12 residues which are located in a cytoplasmic loop between the second and third transmembrane domains.

Last update:

July 1998 / Pattern and text revised.

Technical section:

PROSITE method (with tools and information) covered by this documentation:

DAGK_PROKAR, PS01069; Prokaryotic diacylglycerol kinase signature  (PATTERN)
Consensus pattern: E-x-[LIVM]-N-[ST]-[SA]-[LIV]-E-x(2)-V-D
Sequences known to belong to this class detected by the pattern: ALL
Other sequence(s) detected in Swiss-Prot: NONE
• Retrieve an alignment of Swiss-Prot true positive hits:
  Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS01069
• Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS01069
• Scan Swiss-Prot/TrEMBL entries against PS01069
• view ligand binding statistics
Matching PDB structures: 2KDC [ALL]

Reference:

1 AuthorsSmith R.L., O'Toole J.F., Maguire M.E., Sanders C.R. II
TitleMembrane topology of Escherichia coli diacylglycerol kinase.
SourceJ. Bacteriol. 176:5459-5465(1994).
PubMed ID8071224

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