PROSITE

	Home ScanProsite ProRule Documents Downloads Links Funding
	PROSITE documentation PDOC00826

The following enzymes have been shown [1,2] to be evolutionary related:

• Acetate kinase (EC 2.7.2.1) (acetokinase) (gene ackA) from bacteria and archaebacteria. This enzyme is involved in the interconversion of acetate and acetyl-CoA via the acetyl phosphate intermediate. In anaerobic growth it provides ATP by catabolyzing acetate phosphate into acetate.
• Butyrate kinase (EC 2.7.2.7) (gene buk). This enzyme is involved in the interconversion of butyrate and butyryl-CoA via the butyryl phosphate intermediate. Butyrate kinase is involved in the acidogenic phase of fermentation.
• Propionate kinase (EC 2.7.2,.) (gene tdcD) from Escherichia coli.

We have developed two signature patterns for these enzymes; the first one is located in the N-terminal section and the second in the central section. Both of them are glycine-rich and could be involved in substrate or ATP-binding.

Last update:

December 2004 / Pattern and text revised.

PROSITE methods (with tools and information) covered by this documentation:

ACETATE_KINASE_1, PS01075; Acetate and butyrate kinases family signature 1  (PATTERN)

• Consensus pattern:
  [LIVMFANT]-[LIVM]-x-[LIVMA]-N-x-G-S-[ST](2)-x-[KE]
• Sequences in UniProtKB/Swiss-Prot known to belong to this class: 416
  • detected by PS01075: 407 (true positives)
  • undetected by PS01075: 9 (8 false negatives and 1 'partial')
• Other sequence(s) in UniProtKB/Swiss-Prot detected by PS01075:
  NONE.
• Retrieve an alignment of UniProtKB/Swiss-Prot true positive hits:
  Clustal format, color, condensed view  /  Clustal format, color  /  Clustal format, plain text  /  Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic distribution of all UniProtKB (Swiss-Prot + TrEMBL) entries matching PS01075
• Retrieve a list of all UniProtKB (Swiss-Prot + TrEMBL) entries matching PS01075
• Scan UniProtKB (Swiss-Prot and/or TrEMBL) entries against PS01075
• View ligand binding statistics of PS01075
• Matching PDB structures: 1G99 1SAZ 1TUU 1TUY ... [ALL]

ACETATE_KINASE_2, PS01076; Acetate and butyrate kinases family signature 2  (PATTERN)

• Consensus pattern:
  [LIVMFATQ]-[LIVMA]-x(2)-H-x-G-x-[GT]-x-[ST]-[LIVMA]-x-[TAVC]-x(3)-G
• Sequences in UniProtKB/Swiss-Prot known to belong to this class: 416
  • detected by PS01076: 387 (true positives)
  • undetected by PS01076: 29 (27 false negatives and 2 'partials')
• Other sequence(s) in UniProtKB/Swiss-Prot detected by PS01076:
  4 false positives.
• Retrieve an alignment of UniProtKB/Swiss-Prot true positive hits:
  Clustal format, color, condensed view  /  Clustal format, color  /  Clustal format, plain text  /  Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic distribution of all UniProtKB (Swiss-Prot + TrEMBL) entries matching PS01076
• Retrieve a list of all UniProtKB (Swiss-Prot + TrEMBL) entries matching PS01076
• Scan UniProtKB (Swiss-Prot and/or TrEMBL) entries against PS01076
• View ligand binding statistics of PS01076
• Matching PDB structures: 1G99 1SAZ 1TUU 1TUY ... [ALL]

1	Authors	Grundy F.J., Waters D.A., Allen S.H.G., Henkin T.M.
	Title	Regulation of the Bacillus subtilis acetate kinase gene by CcpA.
	Source	J. Bacteriol. 175:7348-7355(1993).
	PubMed ID	8226682

2	Authors	Oultram J.D., Burr I.D., Elmore M.J., Minton N.P.
	Title	Cloning and sequence analysis of the genes encoding phosphotransbutyrylase and butyrate kinase from Clostridium acetobutylicum NCIMB 8052.
	Source	Gene 131:107-112(1993).
	PubMed ID	8396545

PROSITE is copyright. It is produced by the SIB Swiss Institute Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified. Usage by and for commercial entities requires a license agreement. For information about the licensing scheme send an email to

Prosite License or see: prosite_license.html.


View entry in original PROSITE document format
View entry in raw text format (no links)