PROSITE documentation PDOC00826

Acetate and butyrate kinases family signatures

Description:

The following enzymes have been shown [1,2] to be evolutionary related:

  • Acetate kinase (EC 2.7.2.1) (acetokinase) (gene ackA) from bacteria and archaebacteria. This enzyme is involved in the interconversion of acetate and acetyl-CoA via the acetyl phosphate intermediate. In anaerobic growth it provides ATP by catabolyzing acetate phosphate into acetate.
  • Butyrate kinase (EC 2.7.2.7) (gene buk). This enzyme is involved in the interconversion of butyrate and butyryl-CoA via the butyryl phosphate intermediate. Butyrate kinase is involved in the acidogenic phase of fermentation.
  • Propionate kinase (EC 2.7.2,.) (gene tdcD) from Escherichia coli.

We have developed two signature patterns for these enzymes; the first one is located in the N-terminal section and the second in the central section. Both of them are glycine-rich and could be involved in substrate or ATP-binding.

Last update:

December 2004 / Pattern and text revised.

Technical section:

PROSITE methods (with tools and information) covered by this documentation:

ACETATE_KINASE_1, PS01075Acetate and butyrate kinases family signature 1  (PATTERN)
Consensus pattern: [LIVMFANT]-[LIVM]-x-[LIVMA]-N-x-G-S-[ST](2)-x-[KE]
Sequences known to belong to this class detected by the pattern: ALL
Other sequence(s) detected in Swiss-Prot: NONE.
• Retrieve an alignment of Swiss-Prot true positive hits:
  Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format
Retrieve the sequence logo from the alignment
Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS01075
Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS01075
Scan Swiss-Prot/TrEMBL entries against PS01075
view ligand binding statistics
Matching PDB structures: 1G99 1LP2 1LRG 1SAZ ... [ALL]
ACETATE_KINASE_2, PS01076Acetate and butyrate kinases family signature 2  (PATTERN)
Consensus pattern: [LIVMFATQ]-[LIVMA]-x(2)-H-x-G-x-[GT]-x-[ST]-[LIVMA]-x-[TAVC]-x(3)-G
Sequences known to belong to this class detected by the pattern: ALL
Other sequence(s) detected in Swiss-Prot: NONE.
• Retrieve an alignment of Swiss-Prot true positive hits:
  Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format
Retrieve the sequence logo from the alignment
Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS01076
Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS01076
Scan Swiss-Prot/TrEMBL entries against PS01076
view ligand binding statistics
Matching PDB structures: 1G99 1LP2 1LRG 1SAZ ... [ALL]

References:

1 AuthorsGrundy F.J., Waters D.A., Allen S.H.G., Henkin T.M.
TitleRegulation of the Bacillus subtilis acetate kinase gene by CcpA.
SourceJ. Bacteriol. 175:7348-7355(1993).
PubMed ID8226682
2 AuthorsOultram J.D., Burr I.D., Elmore M.J., Minton N.P.
TitleCloning and sequence analysis of the genes encoding phosphotransbutyrylase and butyrate kinase from Clostridium acetobutylicum NCIMB 8052.
SourceGene 131:107-112(1993).
PubMed ID8396545

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