PROSITE documentation PDOC00837

Adenylate cyclase class-I signatures

Description:

Adenylate cyclase (EC 4.6.1.1) is the enzyme responsible for the synthesis of cAMP from ATP. From sequence data, it has been proposed [1,2] that there are three different classes of adenylate cyclases. Class I cyclases are found in enterobacteria and related Gram-negative bacteria. They are proteins of about 850 residues that consist of two functional domains: a N-terminal catalytic domain and a C-terminal regulatory domain.

As signature patterns we selected two highly conserved regions, the first one is located in the catalytic domain and the second one in the regulatory domain. The second signature includes a conserved histidine which could be phosphorylated by a PTS system IIA enzyme, thus leading to the activation of the cyclase.

Expert(s) to contact by email:

Danchin A.

Last update:

November 1997 / Pattern and text revised.

Technical section:

PROSITE methods (with tools and information) covered by this documentation:

ADENYLATE_CYCLASE_1_1, PS01092Adenylate cyclase class-I signature 1  (PATTERN)
Consensus pattern: E-Y-F-G-[SA](2)-L-W-x-L-Y-K
Sequences known to belong to this class detected by the pattern: ALL
Other sequence(s) detected in Swiss-Prot: NONE.
• Retrieve an alignment of Swiss-Prot true positive hits:
  Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format
Retrieve the sequence logo from the alignment
Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS01092
Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS01092
Scan Swiss-Prot/TrEMBL entries against PS01092
view ligand binding statistics
ADENYLATE_CYCLASE_1_2, PS01093Adenylate cyclase class-I signature 2  (PATTERN)
Consensus pattern: Y-R-N-x-W-[NS]-E-[LIVM]-R-T-L-H-F-x-G
H could be phosphorylated
Sequences known to belong to this class detected by the pattern: ALL
Other sequence(s) detected in Swiss-Prot: NONE.
• Retrieve an alignment of Swiss-Prot true positive hits:
  Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format
Retrieve the sequence logo from the alignment
Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS01093
Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS01093
Scan Swiss-Prot/TrEMBL entries against PS01093
view ligand binding statistics

References:

1 AuthorsDanchin A.
TitlePhylogeny of adenylyl cyclases.
SourceAdv. Second Messenger Phosphoprotein Res. 27:109-162(1993).
PubMed ID8418825
2 AuthorsBarzu O., Danchin A.
TitleAdenylyl cyclases: a heterogeneous class of ATP-utilizing enzymes.
SourceProg. Nucleic Acid Res. Mol. Biol. 49:241-283(1994).
PubMed ID7863008

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