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PROSITE documentation PDOC00844

NNMT/PNMT/TEMT family of methyltransferases signature

Description:

The following cytoplasmic vertebrate methyltransferases are evolutionary related [1,2]:

  • Nicotinamide N-methyltransferase (EC 2.1.1.1) (NNMT). NNMT catalyzes the N- methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for the biotransformation of many drugs and xenobiotic compounds.
  • Phenylethanolamine N-methyltransferase (EC 2.1.1.28) (PNMT). PNMT catalyzes the last step in catecholamine biosynthesis, the conversion of noradrenalin to adrenalin.
  • Indolethylamine N-methyltransferase (EC 2.1.1.49) (INMT).
  • Thioether S-methyltransferase (EC 2.1.1.96) (TEMT). TEMT catalyzes the methylation of dimethyl sulfide into trimethylsulfonium.

These enzymes use S-adenosyl-L-methionine as the methyl donor. They are proteins of 30 to 32 Kd. As a signature pattern we selected the most conserved region, a stretch of 16 residues which are located in the N-terminal section of these enzymes.

Last update:

December 2001 / Pattern and text revised.

Technical section:

PROSITE method (with tools and information) covered by this documentation:

NNMT_PNMT_TEMT, PS01100; NNMT/PNMT/TEMT family of methyltransferases signature  (PATTERN)
Consensus pattern: L-I-D-I-G-S-G-P-T-[IV]-Y-Q-[LV]-L-[SA]-A-C
Sequences known to belong to this class detected by the pattern: ALL
Other sequence(s) detected in Swiss-Prot: NONE
• Retrieve an alignment of Swiss-Prot true positive hits:
  Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS01100
• Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS01100
• Scan Swiss-Prot/TrEMBL entries against PS01100
• view ligand binding statistics
Matching PDB structures: 1HNN 1N7I 1N7J 1YZ3 ... [ALL]

References:

1 AuthorsAksoy S., Szumlanski C.L., Weinshilboum R.M.
TitleHuman liver nicotinamide N-methyltransferase. cDNA cloning, expression, and biochemical characterization.
SourceJ. Biol. Chem. 269:14835-14840(1994).
PubMed ID8182091
2 AuthorsThompson M.A., Moon E., Kim U.-J., Xu J., Siciliano M.J., Weinshilboum R.M.
TitleHuman indolethylamine N-methyltransferase: cDNA cloning and expression, gene cloning, and chromosomal localization.
SourceGenomics 61:285-297(1999).
PubMed ID10552930
DOI10.1006/geno.1999.5960

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