Home | Contact

The following cytoplasmic vertebrate methyltransferases are evolutionary related [1,2]:

• Nicotinamide N-methyltransferase (EC 2.1.1.1) (NNMT). NNMT catalyzes the N- methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for the biotransformation of many drugs and xenobiotic compounds.
• Phenylethanolamine N-methyltransferase (EC 2.1.1.28) (PNMT). PNMT catalyzes the last step in catecholamine biosynthesis, the conversion of noradrenalin to adrenalin.
• Indolethylamine N-methyltransferase (EC 2.1.1.49) (INMT).
• Thioether S-methyltransferase (EC 2.1.1.96) (TEMT). TEMT catalyzes the methylation of dimethyl sulfide into trimethylsulfonium.

These enzymes use S-adenosyl-L-methionine as the methyl donor. They are proteins of 30 to 32 Kd. As a signature pattern we selected the most conserved region, a stretch of 16 residues which are located in the N-terminal section of these enzymes.

December 2001 / Pattern and text revised.

PROSITE method (with tools and information) covered by this documentation:

NNMT_PNMT_TEMT, PS01100; NNMT/PNMT/TEMT family of methyltransferases signature  (PATTERN)

Consensus pattern: L-I-D-I-G-S-G-P-T-[IV]-Y-Q-[LV]-L-[SA]-A-C Sequences known to belong to this class detected by the pattern: ALL Other sequence(s) detected in Swiss-Prot: NONE

• Retrieve an alignment of Swiss-Prot true positive hits:   Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format • Retrieve the sequence logo from the alignment • Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS01100 • Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS01100 • Scan Swiss-Prot/TrEMBL entries against PS01100 • view ligand binding statistics

Matching PDB structures: 1HNN 1N7I 1N7J 1YZ3 ... [ALL]

1	Authors	Aksoy S., Szumlanski C.L., Weinshilboum R.M.
	Title	Human liver nicotinamide N-methyltransferase. cDNA cloning, expression, and biochemical characterization.
	Source	J. Biol. Chem. 269:14835-14840(1994).
	PubMed ID	8182091

2	Authors	Thompson M.A., Moon E., Kim U.-J., Xu J., Siciliano M.J., Weinshilboum R.M.
	Title	Human indolethylamine N-methyltransferase: cDNA cloning and expression, gene cloning, and chromosomal localization.
	Source	Genomics 61:285-297(1999).
	PubMed ID	10552930
	DOI	10.1006/geno.1999.5960

PROSITE is copyright. It is produced by the SIB Swiss Institute Bioinformatics. There are no restrictions on its use by non-profit institutions as long as its content is in no way modified. Usage by and for commercial entities requires a license agreement. For information about the licensing scheme send an email to

Prosite License or see: prosite_license.html.


View entry in original PROSITE document format
View entry in raw text format (no links)

SIB Swiss Institute of Bioinformatics | Disclaimer