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| PROSITE documentation PDOC00856 |
RNA polymerases N / 8 Kd subunits signature
Description:
In eukaryotes, there are three different forms of DNA-dependent RNA
polymerases (EC 2.7.7.6) transcribing different sets of genes. Each class of
RNA polymerase is an assemblage of ten to twelve different polypeptides. In
archaebacteria, there is generally a single form of RNA polymerase which also
consist of an oligomeric assemblage of 10 to 13 polypeptides.
Archaebacterial subunit N (gene rpoN) [1] is a small protein of about 8 Kd, it
is evolutionary related [2] to a 8.3 Kd component shared by all three forms of
eukaryotic RNA polymerases (gene RPB10 in yeast and POLR2J in mammals) as well
as to African swine fever virus protein CP80R [3].
As a signature pattern we selected a conserved region which is located at the
N-terminal extremity of these polymerase subunits; this region contains two
cysteines that binds a zinc ion [4].
Last update:
May 2004 / Text revised.
Technical section:
PROSITE method (with tools and information) covered by this documentation:
| RNA_POL_N_8KD, PS01112; RNA polymerases N / 8 Kd subunits signature (PATTERN) |
| Consensus pattern: |
[LIVMFD]-[LIVMFP]-P-[LIVM]-x-C-[FL]-[ST]-C-G
The 2 C's are zinc ligands |
| Sequences known to belong to this class detected by the pattern: |
ALL |
| Other sequence(s) detected in Swiss-Prot: |
NONE |
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| Matching PDB structures:
1EF4 1I3Q 1I50 1I6H ... [ALL] |
References:
| 1 |
Authors | Langer D., Hain J., Thuriaux P., Zillig W. |
| Title | Transcription in archaea: similarity to that in eucarya. |
| Source | Proc. Natl. Acad. Sci. U.S.A. 92:5768-5772(1995). |
| PubMed ID | 7597027 |
| 2 |
Authors | McKune K., Woychik N.A. |
| Title | Halobacterial S9 operon contains two genes encoding proteins homologous to subunits shared by eukaryotic RNA polymerases I, II, and III. |
| Source | J. Bacteriol. 176:4754-4756(1994). |
| PubMed ID | 8045907 |
| 3 |
Authors | Yanez R.J., Rodriguez J.M., Nogal M.L., Yuste L., Enriquez C., Rodriguez J.F., Vinuela E. |
| Title | Analysis of the complete nucleotide sequence of African swine fever virus. |
| Source | Virology 208:249-278(1995). |
| PubMed ID | 11831707 |
| 4 |
Authors | MacKereth C.D., Arrowsmith C.H., Edwards A.M., McIntosh L.P. |
| Title | Zinc-bundle structure of the essential RNA polymerase subunit RPB10 from Methanobacterium thermoautotrophicum. |
| Source | Proc. Natl. Acad. Sci. U.S.A. 97:6316-6321(2000). |
| PubMed ID | 10841539 |
| DOI | 97/12/6316 |
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