{PDOC00952} {PS01238; GDA1_CD39_NTPASE} {BEGIN} ********************************************************* * GDA1/CD39 family of nucleoside phosphatases signature * ********************************************************* A number of nucleoside diphosphate and triphosphate hydrolases as well as some yet uncharacterized proteins have been found to belong to the same family [1, 2]. This family currently consist of: - Yeast guanosine-diphosphatase (EC 3.6.1.42) (GDPase) (gene GDA1). GDA1 is a golgi integral membrane enzyme that catalyzes the hydrolysis of GDP to GMP. - Potato apyrase (EC 3.6.1.5) (adenosine diphosphatase) (ADPase). Apyrase acts on both ATP and ADP to produce AMP. - Mammalian vascular ATP-diphosphohydrolase (EC 3.6.1.5) (also known as lymphoid cell activation antigen CD39). - Toxoplasma gondii nucleoside-triphosphatases (EC 3.6.1.15) (NTPase). NTPase hydrolyses various nucleoside triphosphates to produce the corresponding nucleoside mono- and diphosphates. This enzyme is secreted into the invaded host cell into the parasitophorous vacuole, a specialized compartment where the parasite intracellulary resides. - Pea nucleoside-triphosphatases (EC 3.6.1.15) (NTPase). - Caenorhabditis elegans hypothetical protein C33H5.14. - Caenorhabditis elegans hypothetical protein R07E4.4. - Yeast chromosome V hypothetical protein YER005w. The above uncharacterized proteins all seem to be membrane-bound. All these proteins share a number of conserved domains. We have selected the best conserved of these domains. It is located in the central section of the proteins. -Consensus pattern: [LIVM]-x-G-x(2)-E-G-x-[FYLS]-x-[FW]-[LIVA]-[TAG]-x-N-[HYF] -Sequences known to belong to this class detected by the pattern: ALL. -Other sequence(s) detected in Swiss-Prot: NONE. -Last update: December 2004 / Pattern and text revised. [ 1] Handa M., Guidotti G. "Purification and cloning of a soluble ATP-diphosphohydrolase (apyrase) from potato tubers (Solanum tuberosum)." Biochem. Biophys. Res. Commun. 218:916-923(1996). PubMed=8579614 [ 2] Vasconcelos E.G., Ferreira S.T., de Carvalho T.M.U., de Souza W., Kettlun A.M., Mancilla M., Valenzuela M.A., Verjovski-Almeida S. "Partial purification and immunohistochemical localization of ATP diphosphohydrolase from Schistosoma mansoni. Immunological cross-reactivities with potato apyrase and Toxoplasma gondii nucleoside triphosphate hydrolase." J. Biol. Chem. 271:22139-22145(1996). PubMed=8703025 -------------------------------------------------------------------------------- PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see https://prosite.expasy.org/prosite_license.html -------------------------------------------------------------------------------- {END}