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PROSITE documentation PDOC00983

Ribonuclease PH signature

Description:

Prokaryotic ribonuclease PH (EC 2.7.7.56) (RNase PH) [1] is a phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates.

RNase PH is a conserved protein of about 240 amino-acid residues. It is evolutionary related to Caenorhabditis elegans hypothetical protein B0564.1.

As a signature pattern, we selected the most highly conserved region which is located in the central part of these proteins.

Last update:

April 2006 / Pattern revised.

Technical section:

PROSITE method (with tools and information) covered by this documentation:

RIBONUCLEASE_PH, PS01277; Ribonuclease PH signature  (PATTERN)
Consensus pattern: [CA]-[DE]-[LIVM](2)-[NQV]-[GTA]-D-[GA]-[SG]-x(2,3)-[TAVLC]-[AT]
Sequences known to belong to this class detected by the pattern: ALL
Other sequence(s) detected in Swiss-Prot: NONE
• Retrieve an alignment of Swiss-Prot true positive hits:
  Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format
• Retrieve the sequence logo from the alignment
• Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS01277
• Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS01277
• Scan Swiss-Prot/TrEMBL entries against PS01277
• view ligand binding statistics
Matching PDB structures: 1OYP 1OYR 1OYS 1R6L ... [ALL]

Reference:

1 AuthorsKelly K.O., Deutscher M.P.
TitleCharacterization of Escherichia coli RNase PH.
SourceJ. Biol. Chem. 267:17153-17158(1992).
PubMed ID1512253

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