{PDOC00989} {PS01287; RTC} {BEGIN} *********************************************** * RNA 3'-terminal phosphate cyclase signature * *********************************************** RNA 3'-terminal phosphate cyclase (EC 6.5.1.4) [1,2] catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing. The reaction catalyzed by the enzyme occurs in three steps: 1) adenylation of the enzyme by ATP; 2) the enzyme acts on RNA-3'terminal phosphate to produce RNA-3'terminal diphosphate adenylate; 3) Release of AMP and cyclisation by a non catalytic nucleophilic attack by the adjacent 2'hydroxyl on the phosphorus in the diester linkage. This enzyme, which has been characterized in human (where there seems to be at least three isozymes) and Escherichia coli (gene rtCA), seems to be taxonomically widespread. It is found in insects, plants, fungi (gene RTC1 in yeast) and in archaebacteria. RNA cyclase is a protein of from 36 to 42 Kd. The best conserved region, which we use as a signature pattern, is a glycine-rich stretch of residues located in the central part of the sequence and which is reminiscent of various ATP, GTP or AMP glycine-rich loops. In this context, the conserved Arg (His in the E.coli enzyme) could be the AMP-binding residue. -Consensus pattern: [RH]-G-x(2)-P-x-G(3)-x-[LIV] -Sequences known to belong to this class detected by the pattern: ALL. -Other sequence(s) detected in Swiss-Prot: NONE. -Expert(s) to contact by email: Filipowicz W.; filipowi@fmi.ch. -Last update: November 1997 / First entry. [ 1] Genschik P., Billy E., Swianiewicz M., Filipowicz W. "The human RNA 3'-terminal phosphate cyclase is a member of a new family of proteins conserved in Eucarya, Bacteria and Archaea." EMBO J. 16:2955-2967(1997). PubMed=9184239; DOI=10.1093/emboj/16.10.2955 [ 2] Filipowicz W., Vicente O. "RNA 3'-terminal phosphate cyclase from HeLa cells." Methods Enzymol. 181:499-510(1990). PubMed=2199762 -------------------------------------------------------------------------------- PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see https://prosite.expasy.org/prosite_license.html -------------------------------------------------------------------------------- {END}