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RuvC (EC 3.1.22.4) [1] is a bacterial nuclease that resolves Holliday junction intermediates in genetic recombination. It cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'terminal phosphate and a 3'terminal hydroxyl group.

It is a small protein of about 20 Kd. It requires and binds a magnesium ion. As a signature pattern we selected a region located in the C-terminal part. This region contains two aspartate residues implicated in the binding of the magnesium ion.

April 2006 / Pattern revised.

PROSITE method (with tools and information) covered by this documentation:

RUVC, PS01321; Crossover junction endodeoxyribonuclease ruvC signature  (PATTERN)

Consensus pattern: G-x-[GA]-x-[AG]-x-K-x-[EQA]-[IVM]-x(16,19)-D-x-[SAVT]-D-[AG]-x-[AGS]-[LIVMCA]-[ACS] The 2 D's are magnesium ligands Sequences known to belong to this class detected by the pattern: ALL Other sequence(s) detected in Swiss-Prot: NONE

• Retrieve an alignment of Swiss-Prot true positive hits:   Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format • Retrieve the sequence logo from the alignment • Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS01321 • Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS01321 • Scan Swiss-Prot/TrEMBL entries against PS01321 • view ligand binding statistics

Matching PDB structures: 1HJR [ALL]

1	Authors	West S.C.
	Title	Processing of recombination intermediates by the RuvABC proteins.
	Source	Annu. Rev. Genet. 31:213-244(1997).
	PubMed ID	9442895
	DOI	10.1146/annurev.genet.31.1.213

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