|PROSITE documentation PDOC01027
Glycosyl hydrolases family 4 signature
It has been shown [1,2,E1] that the following glycosyl hydrolases can be, on
the basis of sequence similarities, classified into a single family:
- Escherichia coli and Bacillus subtilis α-galactosidase (EC 22.214.171.124)
(melibiase) (gene melA).
- Thermotoga maritima α-glucosidase (EC 126.96.36.199) (gene aglA).
- Probable 6-phospho-β-glucosidases (EC 188.8.131.52) (gene celF) from
Escherichia coli and Bacillus subtilis.
- Maltose-6'-phosphate glucosidases (EC 184.108.40.206) (6-phospho-α-D-
glucosidase) from Bacillus subtilis (gene glvG) and Fusobacterium
mortiferum (gene malH).
- Bacillus subtilis protein lplD.
These enzymes require NAD and a divalent ion for their activity. They are
proteins of about 50 Kd. As a signature pattern we selected a conserved region
located in the central section. This region does not contain residues directly
shown to be important for the catalytic activity.
April 2006 / Pattern revised.
PROSITE method (with tools and information) covered by this documentation:
|GLYCOSYL_HYDROL_F4, PS01324; Glycosyl hydrolases family 4 signature (PATTERN)
|Sequences known to belong to this class detected by the pattern:
|Other sequence(s) detected in Swiss-Prot:
|Matching PDB structures:
1UP4 1UP6 1UP7 3FEF [ALL]
||A classification of glycosyl hydrolases based on amino acid sequence similarities.
||Biochem. J. 280:309-316(1991).
||Thompson J., Pikis A., Ruvinov S.B., Henrissat B., Yamamoto H., Sekiguchi J.
||The gene glvA of Bacillus subtilis 168 encodes a metal-requiring, NAD(H)-dependent 6-phospho-alpha-glucosidase. Assignment to family 4 of the glycosylhydrolase superfamily.
||J. Biol. Chem. 273:27347-27356(1998).
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