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| PROSITE documentation PDOC01027 |
Glycosyl hydrolases family 4 signature
Description:
It has been shown [1,2,E1] that the following glycosyl hydrolases can be, on
the basis of sequence similarities, classified into a single family:
- Escherichia coli and Bacillus subtilis α-galactosidase (EC 3.2.1.22)
(melibiase) (gene melA).
- Thermotoga maritima α-glucosidase (EC 3.2.1.20) (gene aglA).
- Probable 6-phospho-β-glucosidases (EC 3.2.1.86) (gene celF) from
Escherichia coli and Bacillus subtilis.
- Maltose-6'-phosphate glucosidases (EC 3.2.1.122) (6-phospho-α-D-
glucosidase) from Bacillus subtilis (gene glvG) and Fusobacterium
mortiferum (gene malH).
- Bacillus subtilis protein lplD.
These enzymes require NAD and a divalent ion for their activity. They are
proteins of about 50 Kd. As a signature pattern we selected a conserved region
located in the central section. This region does not contain residues directly
shown to be important for the catalytic activity.
Expert(s) to contact by email:
Henrissat B.
Last update:
April 2006 / Pattern revised.
Technical section:
PROSITE method (with tools and information) covered by this documentation:
| GLYCOSYL_HYDROL_F4, PS01324; Glycosyl hydrolases family 4 signature (PATTERN) |
| Consensus pattern: |
[PS]-x-[SAC]-x-[LIVMFY](2)-[QN]-x(2)-N-P-x(4)-[TA]-x(9,11)-[KRD]-x-[LIV]-[GN]-x-C
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| Sequences known to belong to this class detected by the pattern: |
ALL |
| Other sequence(s) detected in Swiss-Prot: |
NONE. |
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| Matching PDB structures:
1UP4 1UP6 1UP7 3FEF [ALL] |
References:
| 1 |
Authors | Henrissat B. |
| Title | A classification of glycosyl hydrolases based on amino acid sequence similarities. |
| Source | Biochem. J. 280:309-316(1991). |
| PubMed ID | 1747104 |
| 2 |
Authors | Thompson J., Pikis A., Ruvinov S.B., Henrissat B., Yamamoto H., Sekiguchi J. |
| Title | The gene glvA of Bacillus subtilis 168 encodes a metal-requiring, NAD(H)-dependent 6-phospho-alpha-glucosidase. Assignment to family 4 of the glycosylhydrolase superfamily. |
| Source | J. Biol. Chem. 273:27347-27356(1998). |
| PubMed ID | 9765262 |
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