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It has been shown [1,2,E1] that the following glycosyl hydrolases can be, on the basis of sequence similarities, classified into a single family:

• Escherichia coli and Bacillus subtilis α-galactosidase (EC 3.2.1.22) (melibiase) (gene melA).
• Thermotoga maritima α-glucosidase (EC 3.2.1.20) (gene aglA).
• Probable 6-phospho-β-glucosidases (EC 3.2.1.86) (gene celF) from Escherichia coli and Bacillus subtilis.
• Maltose-6'-phosphate glucosidases (EC 3.2.1.122) (6-phospho-α-D- glucosidase) from Bacillus subtilis (gene glvG) and Fusobacterium mortiferum (gene malH).
• Bacillus subtilis protein lplD.

These enzymes require NAD and a divalent ion for their activity. They are proteins of about 50 Kd. As a signature pattern we selected a conserved region located in the central section. This region does not contain residues directly shown to be important for the catalytic activity.

Henrissat B.

April 2006 / Pattern revised.

PROSITE method (with tools and information) covered by this documentation:

GLYCOSYL_HYDROL_F4, PS01324; Glycosyl hydrolases family 4 signature  (PATTERN)

Consensus pattern: [PS]-x-[SAC]-x-[LIVMFY](2)-[QN]-x(2)-N-P-x(4)-[TA]-x(9,11)-[KRD]-x-[LIV]-[GN]-x-C Sequences known to belong to this class detected by the pattern: ALL Other sequence(s) detected in Swiss-Prot: NONE.

• Retrieve an alignment of Swiss-Prot true positive hits:   Clustal format, color, condensed view  / Clustal format, color  / Clustal format, plain text  / Fasta format • Retrieve the sequence logo from the alignment • Taxonomic tree view of all Swiss-Prot/TrEMBL entries matching PS01324 • Retrieve a list of all Swiss-Prot/TrEMBL entries matching PS01324 • Scan Swiss-Prot/TrEMBL entries against PS01324 • view ligand binding statistics

Matching PDB structures: 1UP4 1UP6 1UP7 3FEF [ALL]

1	Authors	Henrissat B.
	Title	A classification of glycosyl hydrolases based on amino acid sequence similarities.
	Source	Biochem. J. 280:309-316(1991).
	PubMed ID	1747104

2	Authors	Thompson J., Pikis A., Ruvinov S.B., Henrissat B., Yamamoto H., Sekiguchi J.
	Title	The gene glvA of Bacillus subtilis 168 encodes a metal-requiring, NAD(H)-dependent 6-phospho-alpha-glucosidase. Assignment to family 4 of the glycosylhydrolase superfamily.
	Source	J. Biol. Chem. 273:27347-27356(1998).
	PubMed ID	9765262

E1
Source	http://www.uniprot.org/docs/glycosid

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