{PDOC01046} {PS01347; MRAY_1} {PS01348; MRAY_2} {BEGIN} ************************** * MraY family signatures * ************************** Phospho-N-acetylmuramoyl-pentapeptide-transferase (EC 2.7.8.13) (mraY) [1] is a bacterial enzyme responsible for the formation of the first lipid intermediate of the cell wall peptidoglycan synthesis. It catalyzes the formation of undecaprenyl-pyrophosphoryl-N-acetylmuramoyl-pentapeptide from UDP-MurNAc-pentapeptide and undecaprenyl-phosphate. It is an integral membrane protein with probably ten transmembrane domains. It belongs to family 4 of glycosyl transferases. Homologs of mraY have been found in the archaebacteria Methanobacterium thermoautotrophicum and in Arabidopsis thaliana. We developed two patterns for this family of enzyme. The first one is located at the end of the first cytoplasmic loop and the beginning of the second transmembrane domain. The second pattern is located in the third cytoplasmic loop. -Consensus pattern: [KRA]-x(2)-[TIVK]-P-[ST]-[MGA]-[GA]-G-[LIVSA]-x-[LIVMF](2) -Sequences known to belong to this class detected by the pattern: ALL. -Other sequence(s) detected in Swiss-Prot: NONE. -Consensus pattern: [NHS]-x(2)-[NK]-x-[TINAS]-[DN]-G-[ILVM]-D-G-[LM] -Sequences known to belong to this class detected by the pattern: ALL. -Other sequence(s) detected in Swiss-Prot: Haemophilus influenzae rfe, which also belongs to glycosyl transferase family 4. -Expert(s) to contact by email: Bouhss A.; abouhss@pasteur.fr -Last update: April 2006 / Pattern revised. [ 1] Bouhss A., Mengin-Lecreulx D., Le Beller D., Van Heijenoort J. "Topological analysis of the MraY protein catalysing the first membrane step of peptidoglycan synthesis." Mol. Microbiol. 34:576-585(1999). PubMed=10564498 -------------------------------------------------------------------------------- PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see https://prosite.expasy.org/prosite_license.html -------------------------------------------------------------------------------- {END}